UniProt Accession

 PEBB_HUMAN; Q13951; A8K347; Q13124; Q9HCT2 

Genbank Protein ID

 AF294326; BT006862; AK290462; CH471092; CH471092; BC018509; L20298 

Genbank Nucleotide ID

 AAG01553.1; AAP35508.1; BAF83151.1; EAW83067.1; EAW83068.1; AAH18509.1; AAA02868.1 

Protein Name

 Core-binding factor subunit beta 

Protein Synonyms/Alias

 CBF-beta; Polyomavirus enhancer-binding protein 2 beta subunit; PEA2-beta; PEBP2-beta; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit 

Gene Name

 CBFB 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
11	VVPDQRSKFENEEFF	acetylation	[1]
11	VVPDQRSKFENEEFF	ubiquitination	[2]
28	LSRECEIKYTGFRDR	ubiquitination	[2, 3, 4]
94	DLEREAGKVYLKAPM	ubiquitination	[2]
177	QLLAVTGKKTTRP**	ubiquitination	[2, 4]
178	LLAVTGKKTTRP***	ubiquitination	[2]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM- CSF promoters. CBFB enhances DNA binding by RUNX1. 

Sequence Annotation

  

Keyword

 3D-structure; Alternative splicing; Chromosomal rearrangement; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 182 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0003713; F:transcription coactivator activity; IEA:InterPro.
 GO:0048469; P:cell maturation; IEA:Compara.
 GO:0060216; P:definitive hemopoiesis; IEA:Compara.
 GO:0030098; P:lymphocyte differentiation; IEA:Compara.
 GO:0030099; P:myeloid cell differentiation; IEA:Compara.
 GO:0001649; P:osteoblast differentiation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc. 

Interpro

 IPR003417; CBF_beta. 

Pfam

 PF02312; CBF_beta 

SMART

  

PROSITE

  

PRINTS