CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-039770
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 E3 ubiquitin-protein ligase makorin-1 
Protein Synonyms/Alias
  
Gene Name
 MKRN1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
11SFAVQRSKDMVCGICubiquitination[1]
55IRKWRSAKQFESKIIubiquitination[2, 3]
60SAKQFESKIIKSCPEubiquitination[1, 4]
85SEYWVEEKEEKQKLIubiquitination[3, 5, 6]
88WVEEKEEKQKLILKYubiquitination[1]
102YKEAMSNKACRYFDEubiquitination[1]
123FGGNCFYKHAYPDGRubiquitination[1, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Metal-binding; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 216 AA 
Protein Sequence
MELSFAVQRS KDMVCGICME VVYEKANPSE RRFGILSNCN HTYCLKCIRK WRSAKQFESK 60
IIKSCPECRI TSNFVIPSEY WVEEKEEKQK LILKYKEAMS NKACRYFDEG RGSCPFGGNC 120
FYKHAYPDGR REEPQRQKVG TSSRYRAQRR NHFWELIEER ENSNPFDNDE EEVVTFELGE 180
MLLMLLAAGG DDELTDSEDE WDLFHDELED FYDLDL 216 
Gene Ontology
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR026290; Makorin-related.
 IPR000571; Znf_CCCH.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 
Pfam
 PF13639; zf-RING_2 
SMART
 SM00184; RING 
PROSITE
 PS50103; ZF_C3H1
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS