CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001803
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Prothrombin 
Protein Synonyms/Alias
 Coagulation factor II; Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain 
Gene Name
 F2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Bos taurus (Bovine) 
NCBI Taxa ID
 9913 
Lysine Modification
Position
Peptide
Type
References
46QRARRANKGFLEEVRacetylation[1]
54GFLEEVRKGNLERECacetylation[1]
87ATDAFWAKYTACESAacetylation[1]
100SARNPREKLNECLEGacetylation[1]
140WRSRYPHKPEINSTTacetylation[1]
Reference
 [1] Amino-terminal alanine functions in a calcium-specific process essential for membrane binding by prothrombin fragment 1.
 Welsch DJ, Nelsestuen GL.
 Biochemistry. 1988 Jun 28;27(13):4939-45. [PMID: 3167022] 
Functional Description
 Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity). 
Sequence Annotation
 DOMAIN 44 90 Gla.
 DOMAIN 109 187 Kringle 1.
 DOMAIN 214 292 Kringle 2.
 DOMAIN 367 621 Peptidase S1.
 REGION 554 576 High affinity receptor-binding region
 ACT_SITE 409 409 Charge relay system.
 ACT_SITE 465 465 Charge relay system.
 ACT_SITE 571 571 Charge relay system.
 MOD_RES 50 50 4-carboxyglutamate.
 MOD_RES 51 51 4-carboxyglutamate.
 MOD_RES 58 58 4-carboxyglutamate.
 MOD_RES 60 60 4-carboxyglutamate.
 MOD_RES 63 63 4-carboxyglutamate.
 MOD_RES 64 64 4-carboxyglutamate.
 MOD_RES 69 69 4-carboxyglutamate.
 MOD_RES 70 70 4-carboxyglutamate.
 MOD_RES 73 73 4-carboxyglutamate.
 MOD_RES 76 76 4-carboxyglutamate.
 CARBOHYD 120 120 N-linked (GlcNAc...).
 CARBOHYD 144 144 N-linked (GlcNAc...).
 CARBOHYD 419 419 N-linked (GlcNAc...).
 DISULFID 61 66
 DISULFID 91 104
 DISULFID 109 187
 DISULFID 130 170
 DISULFID 158 182
 DISULFID 214 292
 DISULFID 235 275
 DISULFID 263 287
 DISULFID 339 485 Interchain (between light and heavy
 DISULFID 394 410
 DISULFID 539 553
 DISULFID 567 597  
Keyword
 3D-structure; Acute phase; Blood coagulation; Calcium; Cleavage on pair of basic residues; Complete proteome; Direct protein sequencing; Disulfide bond; Gamma-carboxyglutamic acid; Glycoprotein; Hemostasis; Hydrolase; Kringle; Protease; Reference proteome; Repeat; Secreted; Serine protease; Signal; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 625 AA 
Protein Sequence
MARVRGPRLP GCLALAALFS LVHSQHVFLA HQQASSLLQR ARRANKGFLE EVRKGNLERE 60
CLEEPCSREE AFEALESLSA TDAFWAKYTA CESARNPREK LNECLEGNCA EGVGMNYRGN 120
VSVTRSGIEC QLWRSRYPHK PEINSTTHPG ADLRENFCRN PDGSITGPWC YTTSPTLRRE 180
ECSVPVCGQD RVTVEVIPRS GGSTTSQSPL LETCVPDRGR EYRGRLAVTT SGSRCLAWSS 240
EQAKALSKDQ DFNPAVPLAE NFCRNPDGDE EGAWCYVADQ PGDFEYCDLN YCEEPVDGDL 300
GDRLGEDPDP DAAIEGRTSE DHFQPFFNEK TFGAGEADCG LRPLFEKKQV QDQTEKELFE 360
SYIEGRIVEG QDAEVGLSPW QVMLFRKSPQ ELLCGASLIS DRWVLTAAHC LLYPPWDKNF 420
TVDDLLVRIG KHSRTRYERK VEKISMLDKI YIHPRYNWKE NLDRDIALLK LKRPIELSDY 480
IHPVCLPDKQ TAAKLLHAGF KGRVTGWGNR RETWTTSVAE VQPSVLQVVN LPLVERPVCK 540
ASTRIRITDN MFCAGYKPGE GKRGDACEGD SGGPFVMKSP YNNRWYQMGI VSWGEGCDRD 600
GKYGFYTHVF RLKKWIQKVI DRLGS 625 
Gene Ontology
 GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0004252; F:serine-type endopeptidase activity; IEA:Compara.
 GO:0070053; F:thrombospondin receptor activity; IEA:Compara.
 GO:0006953; P:acute-phase response; IEA:UniProtKB-KW.
 GO:0007166; P:cell surface receptor signaling pathway; IEA:Compara.
 GO:0051480; P:cytosolic calcium ion homeostasis; IEA:Compara.
 GO:0042730; P:fibrinolysis; IEA:Compara.
 GO:0048712; P:negative regulation of astrocyte differentiation; IEA:Compara.
 GO:0045861; P:negative regulation of proteolysis; IEA:Compara.
 GO:0030168; P:platelet activation; IEA:Compara.
 GO:0030194; P:positive regulation of blood coagulation; IDA:BHF-UCL.
 GO:0030307; P:positive regulation of cell growth; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0032967; P:positive regulation of collagen biosynthetic process; IEA:Compara.
 GO:0014068; P:positive regulation of phosphatidylinositol 3-kinase cascade; IEA:Compara.
 GO:1900738; P:positive regulation of phospholipase C-activating G-protein coupled receptor signaling pathway; IEA:Compara.
 GO:0001934; P:positive regulation of protein phosphorylation; IEA:Compara.
 GO:2000379; P:positive regulation of reactive oxygen species metabolic process; IEA:Compara.
 GO:0051281; P:positive regulation of release of sequestered calcium ion into cytosol; IEA:Compara.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0008360; P:regulation of cell shape; IEA:Compara. 
Interpro
 IPR017857; Coagulation_fac_subgr_Gla_dom.
 IPR000294; GLA_domain.
 IPR000001; Kringle.
 IPR013806; Kringle-like.
 IPR018056; Kringle_CS.
 IPR001254; Peptidase_S1.
 IPR018114; Peptidase_S1_AS.
 IPR001314; Peptidase_S1A.
 IPR003966; Prothrombin/thrombin.
 IPR018992; Thrombin_light_chain.
 IPR009003; Trypsin-like_Pept_dom. 
Pfam
 PF00594; Gla
 PF00051; Kringle
 PF09396; Thrombin_light
 PF00089; Trypsin 
SMART
 SM00069; GLA
 SM00130; KR
 SM00020; Tryp_SPc 
PROSITE
 PS00011; GLA_1
 PS50998; GLA_2
 PS00021; KRINGLE_1
 PS50070; KRINGLE_2
 PS50240; TRYPSIN_DOM
 PS00134; TRYPSIN_HIS
 PS00135; TRYPSIN_SER 
PRINTS
 PR00722; CHYMOTRYPSIN.
 PR00001; GLABLOOD.
 PR01505; PROTHROMBIN.