UniProt Accession

 STX4_HUMAN; Q12846; Q15525; Q6FHE8 

Genbank Protein ID

 U07158; X85784; AJ000541; AF026007; AF318489; BT007326; CR541806; AK315716; CH471192; BC002436 

Genbank Nucleotide ID

 AAA20967.1; CAA59769.1; CAA04174.1; AAB88810.1; AAG40313.1; AAP35990.1; CAG46605.1; BAG38075.1; EAW52176.1; AAH02436.1 

Protein Name

 Syntaxin-4 

Protein Synonyms/Alias

 Renal carcinoma antigen NY-REN-31 

Gene Name

 STX4 

Gene Synonyms/Alias

 STX4A 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
44	PDEEFFHKVRTIRQT	ubiquitination	[1]
58	TIVKLGNKVQELEKQ	ubiquitination	[1]
91	QNLRDEIKQLGREIR	ubiquitination	[2, 3, 4]
102	REIRLQLKAIEPQKE	ubiquitination	[1]
108	LKAIEPQKEEADENY	ubiquitination	[1, 5]
243	EMINRIEKNILSSAD	ubiquitination	[2, 3, 4]
260	ERGQEHVKTALENQK	ubiquitination	[5]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones. 

Sequence Annotation

 DOMAIN 200 262 t-SNARE coiled-coil homology.
 REGION 154 297 Interaction with CENPF (By similarity).
 MOD_RES 14 14 Phosphoserine.
 MOD_RES 15 15 Phosphoserine.
 MOD_RES 117 117 Phosphoserine.  

Keyword

 Cell membrane; Coiled coil; Complete proteome; Membrane; Neurotransmitter transport; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 297 AA 

Protein Sequence

Gene Ontology

 GO:0016323; C:basolateral plasma membrane; IDA:HGNC.
 GO:0009986; C:cell surface; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0043219; C:lateral loop; IEA:Compara.
 GO:0035749; C:myelin sheath adaxonal region; IEA:Compara.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0031201; C:SNARE complex; IDA:UniProtKB.
 GO:0042581; C:specific granule; IDA:UniProtKB.
 GO:0005802; C:trans-Golgi network; IEA:Compara.
 GO:0005773; C:vacuole; TAS:HGNC.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0006892; P:post-Golgi vesicle-mediated transport; TAS:Reactome. 

Interpro

 IPR006012; Syntaxin/epimorphin_CS.
 IPR006011; Syntaxin_N.
 IPR010989; t-SNARE.
 IPR000727; T_SNARE_dom. 

Pfam

 PF05739; SNARE
 PF00804; Syntaxin 

SMART

 SM00503; SynN
 SM00397; t_SNARE 

PROSITE

 PS00914; SYNTAXIN
 PS50192; T_SNARE 

PRINTS