CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002744
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glyceraldehyde-3-phosphate dehydrogenase 1 
Protein Synonyms/Alias
 NAD-dependent glyceraldehyde-3-phosphate dehydrogenase; GAPDH 
Gene Name
 gapA 
Gene Synonyms/Alias
 gap; BSU33940 
Created Date
 July 27, 2013 
Organism
 Bacillus subtilis (strain 168) 
NCBI Taxa ID
 224308 
Lysine Modification
Position
Peptide
Type
References
167FAKVLNDKFGIKRGMacetylation[1]
193QILDLPHKDYRRARAacetylation[1]
224SLVLPELKGKLNGGAacetylation[1]
332DLAAYIAKKGL****acetylation[1]
Reference
 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 
Functional Description
 More active in catabolism. 
Sequence Annotation
 NP_BIND 12 13 NAD (By similarity).
 REGION 151 153 Glyceraldehyde 3-phosphate binding (By
 REGION 210 211 Glyceraldehyde 3-phosphate binding (By
 ACT_SITE 152 152 Nucleophile (By similarity).
 BINDING 34 34 NAD (By similarity).
 BINDING 78 78 NAD; via carbonyl oxygen (By similarity).
 BINDING 182 182 Glyceraldehyde 3-phosphate (By
 BINDING 197 197 Glyceraldehyde 3-phosphate (By
 BINDING 233 233 Glyceraldehyde 3-phosphate (By
 BINDING 315 315 NAD (By similarity).  
Keyword
 Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 335 AA 
Protein Sequence
MAVKVGINGF GRIGRNVFRA ALNNPEVEVV AVNDLTDANM LAHLLQYDSV HGKLDAEVSV 60
DGNNLVVNGK TIEVSAERDP AKLSWGKQGV EIVVESTGFF TKRADAAKHL EAGAKKVIIS 120
APANEEDITI VMGVNEDKYD AANHDVISNA SCTTNCLAPF AKVLNDKFGI KRGMMTTVHS 180
YTNDQQILDL PHKDYRRARA AAENIIPTST GAAKAVSLVL PELKGKLNGG AMRVPTPNVS 240
LVDLVAELNQ EVTAEEVNAA LKEAAEGDLK GILGYSEEPL VSGDYNGNKN SSTIDALSTM 300
VMEGSMVKVI SWYDNESGYS NRVVDLAAYI AKKGL 335 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IEA:EC.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway. 
Interpro
 IPR020831; GlycerAld/Erythrose_P_DH.
 IPR020830; GlycerAld_3-P_DH_AS.
 IPR020829; GlycerAld_3-P_DH_cat.
 IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
 IPR006424; Glyceraldehyde-3-P_DH_1.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF02800; Gp_dh_C
 PF00044; Gp_dh_N 
SMART
 SM00846; Gp_dh_N 
PROSITE
 PS00071; GAPDH 
PRINTS
 PR00078; G3PDHDRGNASE.