CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024175
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kelch-like ECH-associated protein 1 
Protein Synonyms/Alias
 Cytosolic inhibitor of Nrf2; INrf2 
Gene Name
 Keap1 
Gene Synonyms/Alias
 Inrf2; Kiaa0132 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
131SIEGIHPKVMERLIEacetylation[1]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome (By similarity). 
Sequence Annotation
 DOMAIN 77 149 BTB.
 DOMAIN 184 286 BACK.
 REPEAT 327 372 Kelch 1.
 REPEAT 373 423 Kelch 2.
 REPEAT 424 470 Kelch 3.
 REPEAT 471 517 Kelch 4.
 REPEAT 519 564 Kelch 5.
 REPEAT 565 611 Kelch 6.
 MOTIF 301 310 Nuclear export signal (By similarity).  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Kelch repeat; Nucleus; Reference proteome; Repeat; Transcription; Transcription regulation; Ubl conjugation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 624 AA 
Protein Sequence
MQPEPKLSGA PRSSQFLPLW SKCPEGAGDA VMYASTECKA EVTPSQDGNR TFSYTLEDHT 60
KQAFGVMNEL RLSQQLCDVT LQVKYEDIPA AQFMAHKVVL ASSSPVFKAM FTNGLREQGM 120
EVVSIEGIHP KVMERLIEFA YTASISVGEK CVLHVMNGAV MYQIDSVVRA CSDFLVQQLD 180
PSNAIGIANF AEQIGCTELH QRAREYIYMH FGEVAKQEEF FNLSHCQLAT LISRDDLNVR 240
CESEVFHACI DWVKYDCPQR RFYVQALLRA VRCHALTPRF LQTQLQKCEI LQADARCKDY 300
LVQIFQELTL HKPTQAVPCR APKVGRLIYT AGGYFRQSLS YLEAYNPSNG SWLRLADLQV 360
PRSGLAGCVV GGLLYAVGGR NNSPDGNTDS SALDCYNPMT NQWSPCASMS VPRNRIGVGV 420
IDGHIYAVGG SHGCIHHSSV ERYEPERDEW HLVAPMLTRR IGVGVAVLNR LLYAVGGFDG 480
TNRLNSAECY YPERNEWRMI TPMNTIRSGA GVCVLHNCIY AAGGYDGQDQ LNSVERYDVE 540
TETWTFVAPM RHHRSALGIT VHQGKIYVLG GYDGHTFLDS VECYDPDSDT WSEVTRMTSG 600
RSGVGVAVTM EPCRKQIDQQ NCTC 624 
Gene Ontology
 GO:0005813; C:centrosome; IEA:Compara.
 GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
 GO:0005783; C:endoplasmic reticulum; IDA:MGI.
 GO:0030496; C:midbody; IEA:Compara.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0071353; P:cellular response to interleukin-4; IDA:MGI.
 GO:0001701; P:in utero embryonic development; IMP:MGI.
 GO:0010499; P:proteasomal ubiquitin-independent protein catabolic process; ISS:UniProtKB.
 GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
 GO:0045604; P:regulation of epidermal cell differentiation; IMP:MGI.
 GO:0006355; P:regulation of transcription, DNA-dependent; IMP:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR015916; Gal_Oxidase_b-propeller.
 IPR017096; Kelch-like_gigaxonin.
 IPR006652; Kelch_1. 
Pfam
 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 
SMART
 SM00875; BACK
 SM00225; BTB
 SM00612; Kelch 
PROSITE
 PS50097; BTB 
PRINTS