CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002889
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein RecA 
Protein Synonyms/Alias
 Recombinase A; Endonuclease PI-MtuI; Mtu RecA intein 
Gene Name
 recA 
Gene Synonyms/Alias
 Rv2737c; MT2806; MTV002.02c 
Created Date
 July 27, 2013 
Organism
 Mycobacterium tuberculosis 
NCBI Taxa ID
 1773 
Lysine Modification
Position
Peptide
Type
References
762DVADEIEKKIKEKLGpupylation[1]
Reference
 [1] Prokaryotic ubiquitin-like protein (Pup) proteome of Mycobacterium tuberculosis [corrected] .
 Festa RA, McAllister F, Pearce MJ, Mintseris J, Burns KE, Gygi SP, Darwin KH.
 PLoS One. 2010 Jan 6;5(1):e8589. [PMID: 20066036] 
Functional Description
 Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage. 
Sequence Annotation
 DOMAIN 366 508 DOD-type homing endonuclease.
 NP_BIND 67 74 ATP (By similarity).
 CROSSLNK 762 762 Isoglutamyl lysine isopeptide (Lys-Gln)  
Keyword
 3D-structure; ATP-binding; Autocatalytic cleavage; Complete proteome; Cytoplasm; DNA damage; DNA recombination; DNA repair; DNA-binding; Endonuclease; Hydrolase; Intron homing; Isopeptide bond; Nuclease; Nucleotide-binding; Protein splicing; Reference proteome; SOS response; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 790 AA 
Protein Sequence
MTQTPDREKA LELAVAQIEK SYGKGSVMRL GDEARQPISV IPTGSIALDV ALGIGGLPRG 60
RVIEIYGPES SGKTTVALHA VANAQAAGGV AAFIDAEHAL DPDYAKKLGV DTDSLLVSQP 120
DTGEQALEIA DMLIRSGALD IVVIDSVAAL VPRAELEGEM GDSHVGLQAR LMSQALRKMT 180
GALNNSGTTA IFINQLRDKI GVMFGSPETT TGGKALKFYA SVRMDVRRVE TLKDGTNAVG 240
NRTRVKVVKN KCLAEGTRIF DPVTGTTHRI EDVVDGRKPI HVVAAAKDGT LHARPVVSWF 300
DQGTRDVIGL RIAGGAIVWA TPDHKVLTEY GWRAAGELRK GDRVAQPRRF DGFGDSAPIP 360
ADHARLLGYL IGDGRDGWVG GKTPINFINV QRALIDDVTR IAATLGCAAH PQGRISLAIA 420
HRPGERNGVA DLCQQAGIYG KLAWEKTIPN WFFEPDIAAD IVGNLLFGLF ESDGWVSREQ 480
TGALRVGYTT TSEQLAHQIH WLLLRFGVGS TVRDYDPTQK RPSIVNGRRI QSKRQVFEVR 540
ISGMDNVTAF AESVPMWGPR GAALIQAIPE ATQGRRRGSQ ATYLAAEMTD AVLNYLDERG 600
VTAQEAAAMI GVASGDPRGG MKQVLGASRL RRDRVQALAD ALDDKFLHDM LAEELRYSVI 660
REVLPTRRAR TFDLEVEELH TLVAEGVVVH NCSPPFKQAE FDILYGKGIS REGSLIDMGV 720
DQGLIRKSGA WFTYEGEQLG QGKENARNFL VENADVADEI EKKIKEKLGI GAVVTDDPSN 780
DGVLPAPVDF 790 
Gene Ontology
 GO:0005829; C:cytosol; IDA:MTBBASE.
 GO:0005524; F:ATP binding; IDA:MTBBASE.
 GO:0003684; F:damaged DNA binding; IEA:HAMAP.
 GO:0008094; F:DNA-dependent ATPase activity; IDA:MTBBASE.
 GO:0004520; F:endodeoxyribonuclease activity; IDA:MTBBASE.
 GO:0000287; F:magnesium ion binding; IDA:MTBBASE.
 GO:0030145; F:manganese ion binding; IDA:MTBBASE.
 GO:0000150; F:recombinase activity; IDA:MTBBASE.
 GO:0003697; F:single-stranded DNA binding; IDA:MTBBASE.
 GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
 GO:0006314; P:intron homing; IEA:UniProtKB-KW.
 GO:0000725; P:recombinational repair; IMP:MTBBASE.
 GO:0046677; P:response to antibiotic; IEP:MTBBASE.
 GO:0009432; P:SOS response; IEA:HAMAP.
 GO:0042148; P:strand invasion; IDA:MTBBASE.
 GO:0009650; P:UV protection; IMP:MTBBASE. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR013765; DNA_recomb/repair_RecA.
 IPR020584; DNA_recomb/repair_RecA_CS.
 IPR020588; DNA_recomb_RecA/RadB_ATP-bd.
 IPR003586; Hint_dom_C.
 IPR003587; Hint_dom_N.
 IPR027434; Homing_endonucl.
 IPR006142; INTEIN.
 IPR004042; Intein_endonuc.
 IPR006141; Intein_splice_site.
 IPR027417; P-loop_NTPase.
 IPR023400; RecA_C.
 IPR020587; RecA_monomer-monomer_interface. 
Pfam
 PF00154; RecA 
SMART
 SM00382; AAA
 SM00305; HintC
 SM00306; HintN 
PROSITE
 PS50818; INTEIN_C_TER
 PS50819; INTEIN_ENDONUCLEASE
 PS50817; INTEIN_N_TER
 PS00321; RECA_1
 PS50162; RECA_2
 PS50163; RECA_3 
PRINTS
 PR00379; INTEIN.
 PR00142; RECA.