UniProt Accession

 GREB_ECOLI; P30128; P78114; Q2M771 

Genbank Protein ID

 U81522; U18997; U00096; AP009048 

Genbank Nucleotide ID

 AAB39763.1; AAA58203.1; AAC76431.2; BAE77885.1 

Protein Name

 Transcription elongation factor GreB 

Protein Synonyms/Alias

 Transcript cleavage factor GreB 

Gene Name

 greB 

Gene Synonyms/Alias

 b3406; JW3369 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
13	VTREGYEKLKQELNY	acetylation	[1]
15	REGYEKLKQELNYLW	acetylation	[1]
52	NADYQYNKKRLREID	acetylation	[1]
73	TKCLENLKIVDYSPQ	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreB releases sequences of up to 9 nucleotides in length. 

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; DNA-binding; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 158 AA 

Protein Sequence

Gene Ontology

 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0006352; P:DNA-dependent transcription, initiation; IDA:EcoCyc.
 GO:0031439; P:positive regulation of mRNA cleavage; IMP:EcoCyc.
 GO:0032784; P:regulation of DNA-dependent transcription, elongation; IDA:EcoCyc. 

Interpro

 IPR018151; TF_GreA/GreB_CS.
 IPR023459; Tscrpt_elong_fac_GreA/B.
 IPR001437; Tscrpt_elong_fac_GreA/B_C.
 IPR022691; Tscrpt_elong_fac_GreA/B_N.
 IPR006358; Tscrpt_elong_fac_GreB. 

Pfam

 PF01272; GreA_GreB
 PF03449; GreA_GreB_N 

SMART

  

PROSITE

 PS00829; GREAB_1
 PS00830; GREAB_2 

PRINTS