CPLM-019534

Genbank Nucleotide ID

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
26	MQLMNDKKLMSSLPN	ubiquitination	[1]
67	TLNGSTEKRSAELPD	ubiquitination	[1]
91	EKLYVPVKEYPDFNF	ubiquitination	[1, 2, 3, 4]
111	GPRGLTAKQLEAETG	ubiquitination	[1, 5]
191	EGEDSLKKMQLMELA	ubiquitination	[1]
210	TYRDANIKSPALAFS	ubiquitination	[1]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]

Functional Description

RNA-binding protein that plays a central role in myelinization. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia. Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor (By similarity).

DOMAIN 87 153 KH.
MOTIF 276 279 SH3-binding.
MOTIF 324 330 Nuclear localization signal (By
MOD_RES 242 242 Asymmetric dimethylarginine; by CARM1.

3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Developmental protein; Differentiation; Methylation; mRNA processing; mRNA splicing; mRNA transport; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; RNA-binding; SH3-binding; Translation regulation; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
GO:0042759; P:long-chain fatty acid biosynthetic process; IEA:Compara.
GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
GO:0042692; P:muscle cell differentiation; IEA:Compara.
GO:0042552; P:myelination; IEA:Compara.
GO:0010628; P:positive regulation of gene expression; IEA:Compara.
GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
GO:0007286; P:spermatid development; IEA:Compara.
GO:0001570; P:vasculogenesis; IEA:Compara.

IPR004087; KH_dom.
IPR004088; KH_dom_type_1.