UniProt Accession

 DHX15_MOUSE; O35286; Q99L91 

Genbank Protein ID

 AF017153; BC003745 

Genbank Nucleotide ID

 AAC36129.1 

Protein Name

 Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 

Protein Synonyms/Alias

 DEAH box protein 15 

Gene Name

 Dhx15 

Gene Synonyms/Alias

 Ddx15; Deah9 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
17	GEDYPSGKKRAGTDG	acetylation	[1, 2, 3]
132	PRYYDILKKRLQLPV	ubiquitination	[4]
234	SSAKTILKYMTDGML	acetylation	[1]
488	CFRLYTEKAYKTEMQ	ubiquitination	[4]
491	LYTEKAYKTEMQDNT	ubiquitination	[4]
516	SVVLQLKKLGIDDLV	ubiquitination	[4]
754	IRTCTDIKPEWLVKI	acetylation	[1, 2]
786	QLDRIIAKLQSKEYS	ubiquitination	[4]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA (By similarity). 

Sequence Annotation

 DOMAIN 147 313 Helicase ATP-binding.
 DOMAIN 338 518 Helicase C-terminal.
 NP_BIND 160 167 ATP (By similarity).
 MOTIF 260 263 DEAH box.
 MOD_RES 132 132 N6-acetyllysine (By similarity).
 MOD_RES 488 488 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; Helicase; Hydrolase; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 795 AA 

Protein Sequence

Gene Ontology

 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0005689; C:U12-type spliceosomal complex; ISS:HGNC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 

Interpro

 IPR003593; AAA+_ATPase.
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR002464; DNA/RNA_helicase_DEAH_CS.
 IPR011709; DUF1605.
 IPR007502; Helicase-assoc_dom.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00270; DEAD
 PF04408; HA2
 PF00271; Helicase_C
 PF07717; OB_NTP_bind 

SMART

 SM00382; AAA
 SM00487; DEXDc
 SM00847; HA2
 SM00490; HELICc 

PROSITE

 PS00690; DEAH_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 

PRINTS