UniProt Accession

 UBC6_YEAST; P33296; D3DM07 

Genbank Protein ID

 X73234; U18839; BK006939 

Genbank Nucleotide ID

 CAA51706.1; AAB64655.1; DAA07761.1 

Protein Name

 Ubiquitin-conjugating enzyme E2 6 

Protein Synonyms/Alias

 Ubiquitin carrier protein UBC6; Ubiquitin-protein ligase UBC6 

Gene Name

 UBC6 

Gene Synonyms/Alias

 DOA2; YER100W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
67	FPSDYPYKPPAIRMI	ubiquitination	[1]
151	TFQNVRFKLIFPEVV	ubiquitination	[1, 2]
167	ENVETLEKRKLDEGD	ubiquitination	[1]
169	VETLEKRKLDEGDAA	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Cognate E2 conjugating enzyme for the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains. 

Sequence Annotation

 ACT_SITE 87 87 Glycyl thioester intermediate.
 MOD_RES 139 139 Phosphoserine.
 MOD_RES 178 178 Phosphothreonine.  

Keyword

 ATP-binding; Complete proteome; Endoplasmic reticulum; Ligase; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 250 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IDA:SGD.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:SGD.
 GO:0030433; P:ER-associated protein catabolic process; IMP:SGD.
 GO:0006513; P:protein monoubiquitination; TAS:SGD.
 GO:0000209; P:protein polyubiquitination; TAS:SGD. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS