CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-041105
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hexokinase 1, isoform CRA_f 
Protein Synonyms/Alias
 Hexokinase-1 
Gene Name
 Hk1 
Gene Synonyms/Alias
 mCG_127656 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
314LILVKMAKESLLFEGubiquitination[1]
332PELLTRGKFTTSDVAubiquitination[1]
345VAAIETDKEGVQNAKacetylation[2]
345VAAIETDKEGVQNAKubiquitination[1]
417GVDGSLYKMHPQYSRubiquitination[1]
450LSESGSGKGAAMVTAubiquitination[1]
487KQALMEVKKKLRSEMubiquitination[1]
509TNSRATVKMLPSYVRubiquitination[1]
623ITWTKGFKATDCVGHubiquitination[1]
762NILIDFTKKGFLFRGubiquitination[1]
776GQISEPLKTRGIFETubiquitination[1]
827TVCGVVSKRAAQLCGubiquitination[1]
843GMAAVVEKIRENRGLubiquitination[1]
879RIMHQTVKELSPKCTacetylation[3]
884TVKELSPKCTVSFLLubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Glycolysis; Kinase; Nucleotide-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 917 AA 
Protein Sequence
MDSEHCPSVP CGGAGAWEAR IDKYLYAMRL SDEILIDILT RFKKEMKNGL SRDYNPTASV 60
KMLPTFVRSI PDGSEKGDFI ALDLGGSSFR ILRVQVNHEK SQNVSMESEV YDTPENIVHG 120
SGSQLFDHVA ECLGDFMEKR KIKDKKLPVG FTFSFPCRQS KIDEAVLITW TKRFKASGVE 180
GADVVKLLNK AIKKRGDYDA NIVAVVNDTV GTMMTCGYDD QQCEVGLIIG TGTNACYMEE 240
LRHIDLVEGD EGRMCINTEW GAFGDDGSLE DIRTEFDREL DRGSLNPGKQ LFEKMVSGMY 300
MGELVRLILV KMAKESLLFE GRITPELLTR GKFTTSDVAA IETDKEGVQN AKEILTRLGV 360
EPSHDDCVSV QHVCTIVSFR SANLVAATLG AILNRLRDNK GTPRLRTTVG VDGSLYKMHP 420
QYSRRFHKTL RRLVPDSDVR FLLSESGSGK GAAMVTAVAY RLAEQHRQIE ETLSHFRLSK 480
QALMEVKKKL RSEMEMGLRK ETNSRATVKM LPSYVRSIPD GTEHGDFLAL DLGGTNFRVL 540
LVKIRSGKKR TVEMHNKIYS IPLEIMQGTG DELFDHIVSC ISDFLDYMGI KGPRMPLGFT 600
FSFPCKQTSL DCGILITWTK GFKATDCVGH DVATLLRDAV KRREEFDLDV VAVVNDTVGT 660
MMTCAYEEPS CEIGLIVGTG SNACYMEEMK NVEMVEGNQG QMCINMEWGA FGDNGCLDDI 720
RTDFDKVVDE YSLNSGKQRF EKMISGMYLG EIVRNILIDF TKKGFLFRGQ ISEPLKTRGI 780
FETKFLSQIE SDRLALLQVR AILQQLGLNS TCDDSILVKT VCGVVSKRAA QLCGAGMAAV 840
VEKIRENRGL DHLNVTVGVD GTLYKLHPHF SRIMHQTVKE LSPKCTVSFL LSEDGSGKGA 900
ALITAVGVRL RGDPTNA 917 
Gene Ontology
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0045121; C:membrane raft; IDA:MGI.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008865; F:fructokinase activity; IBA:RefGenome.
 GO:0004340; F:glucokinase activity; IBA:RefGenome.
 GO:0019158; F:mannokinase activity; IBA:RefGenome.
 GO:0001678; P:cellular glucose homeostasis; IBA:RefGenome.
 GO:0006096; P:glycolysis; TAS:MGI. 
Interpro
 IPR001312; Hexokinase.
 IPR022673; Hexokinase_C.
 IPR019807; Hexokinase_CS.
 IPR022672; Hexokinase_N. 
Pfam
 PF00349; Hexokinase_1
 PF03727; Hexokinase_2 
SMART
  
PROSITE
 PS00378; HEXOKINASES 
PRINTS
 PR00475; HEXOKINASE.