UniProt Accession

 ENO_SALTY; P64076; Q8XGP6 

Genbank Protein ID

 AE006468 

Genbank Nucleotide ID

 AAL21832.1 

Protein Name

 Enolase 

Protein Synonyms/Alias

 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase 

Gene Name

 eno 

Gene Synonyms/Alias

 STM2952 

Created Date

 July 27, 2013 

Organism

 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 

NCBI Taxa ID

 99287 

Lysine Modification

Position	Peptide	Type	References
405	SRSDRVAKYNQLIRI	acetylation	[1]
426	KAPYNGRKEIKGQA*	acetylation	[1]

Reference

 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 

Functional Description

 Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis. It is also a component of the RNA degradosome, a multi-enzyme complex involved in RNA processing and messenger RNA degradation. Its interaction with RNase E is important for the turnover of mRNA, in particular on transcripts encoding enzymes of energy-generating metabolic routes. Its presence in the degradosome is required for the response to excess phosphosugar. May play a regulatory role in the degradation of specific RNAs, such as ptsG mRNA, therefore linking cellular metabolic status with post-translational gene regulation (By similarity). 

Sequence Annotation

 REGION 369 372 Substrate binding (By similarity).
 ACT_SITE 209 209 Proton donor (By similarity).
 ACT_SITE 342 342 Proton acceptor (By similarity).
 METAL 246 246 Magnesium (By similarity).
 METAL 290 290 Magnesium (By similarity).
 METAL 317 317 Magnesium (By similarity).
 BINDING 159 159 Substrate (By similarity).
 BINDING 168 168 Substrate (By similarity).
 BINDING 290 290 Substrate (By similarity).
 BINDING 317 317 Substrate (By similarity).
 BINDING 342 342 Substrate (covalent); in inhibited form
 BINDING 393 393 Substrate (By similarity).
 MOD_RES 284 284 Phosphotyrosine (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Cytoskeleton; Glycolysis; Lyase; Magnesium; Metal-binding; Phosphoprotein; Reference proteome; Secreted. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 432 AA 

Protein Sequence

Gene Ontology

 GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0004634; F:phosphopyruvate hydratase activity; IEA:HAMAP.
 GO:0006096; P:glycolysis; IEA:HAMAP. 

Interpro

 IPR000941; Enolase.
 IPR020810; Enolase_C.
 IPR020809; Enolase_CS.
 IPR020811; Enolase_N. 

Pfam

 PF00113; Enolase_C
 PF03952; Enolase_N 

SMART

  

PROSITE

 PS00164; ENOLASE 

PRINTS

 PR00148; ENOLASE.