UniProt Accession

 DHYS_YEAST; P38791; D3DL17 

Genbank Protein ID

 D78185; U00061; AY558282; BK006934 

Genbank Nucleotide ID

 BAA11253.1; AAB68377.1; AAS56608.1; DAA06761.1 

Protein Name

 Deoxyhypusine synthase 

Protein Synonyms/Alias

 DHS 

Gene Name

 DYS1 

Gene Synonyms/Alias

 YHR068W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
37	VQGIDYSKPEATNMR	acetylation	[1]
78	SMRSWRGKHIDELDD	acetylation	[1]
88	DELDDHEKKGCFDEE	acetylation	[1]
89	ELDDHEKKGCFDEEG	ubiquitination	[2]
157	YLGEFALKGKSLRDQ	ubiquitination	[2]
231	KMIDRFGKEINDESS	acetylation	[1]
286	DIVGDIRKINSMSMA	ubiquitination	[2]
350	DEAVSWGKIKAEAKS	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. 

Sequence Annotation

 NP_BIND 108 112 NAD (By similarity).
 NP_BIND 134 136 NAD (By similarity).
 NP_BIND 329 330 NAD (By similarity).
 NP_BIND 363 364 NAD (By similarity).
 REGION 139 140 Spermidine binding (By similarity).
 REGION 335 337 Spermidine binding (By similarity).
 REGION 344 350 Spermidine binding (By similarity).
 ACT_SITE 350 350 Nucleophile (By similarity).
 BINDING 140 140 NAD (By similarity).
 BINDING 257 257 NAD (By similarity).
 BINDING 262 262 Spermidine (By similarity).
 BINDING 304 304 NAD; via amide nitrogen (By similarity).
 BINDING 309 309 Spermidine (By similarity).  

Keyword

 Complete proteome; Hypusine biosynthesis; NAD; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 387 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0034038; F:deoxyhypusine synthase activity; IEA:EC.
 GO:0016765; F:transferase activity, transferring alkyl or aryl (other than methyl) groups; IDA:SGD.
 GO:0050983; P:deoxyhypusine biosynthetic process from spermidine; IEA:EC.
 GO:0008612; P:peptidyl-lysine modification to hypusine; IDA:SGD. 

Interpro

 IPR002773; Deoxyhypusine_synthase. 

Pfam

 PF01916; DS 

SMART

  

PROSITE

  

PRINTS