| Tag | Content |
|---|
| CPLM ID | CPLM-003295 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Poly(A) polymerase I |
Protein Synonyms/Alias | PAP I; Plasmid copy number protein |
Gene Name | pcnB |
Gene Synonyms/Alias | b0143; JW5808 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 50 | DISENALKVMYRLNK | acetylation | [1] | | 57 | KVMYRLNKAGYEAWL | acetylation | [1] | | 185 | RDYVGGMKDLKDGVI | acetylation | [1] | | 218 | RAVRFAAKLGMRISP | acetylation | [1] | | 252 | RLFEESLKLLQAGYG | acetylation | [1] | | 300 | RIIEQVLKNTDTRIH | acetylation | [1] | | 390 | RQGKRAWKLLEHPKF | acetylation | [1] | | 396 | WKLLEHPKFRAAYDL | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control. Rho-independent transcription terminators may serve as polyadenylation signals. Seems to be involved in plasmid copy number control. |
Sequence Annotation | ACT_SITE 80 80 By similarity.
ACT_SITE 82 82 By similarity.
ACT_SITE 162 162 By similarity. |
Keyword | ATP-binding; Cell inner membrane; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Membrane; mRNA processing; Nucleotide-binding; Plasmid copy control; Reference proteome; RNA-binding; Transcription; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 465 AA |
Protein Sequence | MFTRVANFCR KVLSREESEA EQAVARPQVT VIPREQHAIS RKDISENALK VMYRLNKAGY 60
EAWLVGGGVR DLLLGKKPKD FDVTTNATPE QVRKLFRNCR LVGRRFRLAH VMFGPEIIEV 120
ATFRGHHEGN VSDRTTSQRG QNGMLLRDNI FGSIEEDAQR RDFTINSLYY SVADFTVRDY 180
VGGMKDLKDG VIRLIGNPET RYREDPVRML RAVRFAAKLG MRISPETAEP IPRLATLLND 240
IPPARLFEES LKLLQAGYGY ETYKLLCEYH LFQPLFPTIT RYFTENGDSP MERIIEQVLK 300
NTDTRIHNDM RVNPAFLFAA MFWYPLLETA QKIAQESGLT YHDAFALAMN DVLDEACRSL 360
AIPKRLTTLT RDIWQLQLRM SRRQGKRAWK LLEHPKFRAA YDLLALRAEV ERNAELQRLV 420
KWWGEFQVSA PPDQKGMLNE LDEEPSPRRR TRRPRKRAPR REGTA 465 |
Gene Ontology | GO:0005737; C:cytoplasm; IDA:EcoliWiki. GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0004652; F:polynucleotide adenylyltransferase activity; IDA:EcoliWiki. GO:0003723; F:RNA binding; IMP:EcoliWiki. GO:0006378; P:mRNA polyadenylation; IMP:EcoliWiki. GO:0006276; P:plasmid maintenance; IMP:EcoliWiki. GO:0009451; P:RNA modification; IDA:EcoliWiki. GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. |
Interpro | |
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