UniProt Accession

 VPS9_YEAST; P54787; D6W0I8 

Genbank Protein ID

 U50142; U20373; Z46660; BK006946 

Genbank Nucleotide ID

 AAC49314.1; AAA62233.1; CAA86640.1; DAA09802.1 

Protein Name

 Vacuolar protein sorting-associated protein 9 

Protein Synonyms/Alias

 Vacuolar protein-targeting protein 9 

Gene Name

 VPS9 

Gene Synonyms/Alias

 VPT9; YML097C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
28	QANGNPDKDVKFKKD	ubiquitination	[1]
211	KFVHLASKELGKINR	acetylation	[2]
244	FGLLKHTKLEQNGAD	ubiquitination	[1]
387	PTQEEALKSEQSNKE	ubiquitination	[1, 3]
393	LKSEQSNKEEDVSSL	ubiquitination	[1, 3]
402	EDVSSLIKKIEENER	ubiquitination	[1]
403	DVSSLIKKIEENERK	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Required for vacuolar protein sorting; may be required for the consumption of transport vesicles containing vacuolar protein precursors. May bind a Rab GTPase such as VPS21. 

Sequence Annotation

 DOMAIN 170 312 VPS9.
 DOMAIN 408 451 CUE.
 MOTIF 229 237 GBH motif I.
 MOTIF 249 257 GBH motif II.
 MOTIF 287 295 GBH motif III.
 MOD_RES 375 375 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Phosphoprotein; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 451 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:SGD.
 GO:0043130; F:ubiquitin binding; IDA:SGD.
 GO:0006895; P:Golgi to endosome transport; IMP:SGD.
 GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; IMP:SGD.
 GO:0036010; P:protein localization to endosome; IGI:SGD.
 GO:0006623; P:protein targeting to vacuole; IMP:SGD.
 GO:0000011; P:vacuole inheritance; IMP:SGD. 

Interpro

 IPR003892; CUE.
 IPR009060; UBA-like.
 IPR003123; VPS9.
 IPR013995; VPS9_subgr. 

Pfam

 PF02845; CUE
 PF02204; VPS9 

SMART

 SM00546; CUE
 SM00167; VPS9 

PROSITE

 PS51140; CUE
 PS51205; VPS9 

PRINTS