CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020605
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 SNW domain-containing protein 1 
Protein Synonyms/Alias
 Nuclear protein SkiP; Ski-interacting protein 
Gene Name
 Snw1 
Gene Synonyms/Alias
 Skiip 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
153KTRVALEKSVSQKVAacetylation[1]
158LEKSVSQKVAAAMPVacetylation[1]
170MPVRAADKLAPAQYIacetylation[1, 2, 3]
193VAFNSGAKQRVIRMVubiquitination[4]
339RERRAGIKTHVEKEDacetylation[1]
441DQAWRGGKDMAQSIYacetylation[1]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Involved in transcriptional regulation. Modulates TGF- beta-mediated transcription via association with SMAD proteins, MYOD1-mediated transcription via association with PABPN1, RB1- mediated transcriptional repression, and retinoid-X receptor (RXR)- and vitamin D receptor (VDR)-dependent gene transcription in a cell line-specific manner probably involving coactivators NCOA1 and GRIP1. Is involved in NOTCH1-mediated transcriptional activation. Binds to multimerized forms of Notch intracellular domain (NICD) and is proposed to recruit transcriptional coactivators such as MAML1 to form an intermediate preactivation complex which associates with DNA-bound CBF-1/RBPJ to form a transcriptional activation complex by releasing SNW1 and redundant NOTCH1 NICD. Functions as a splicing factor in pre-mRNA splicing. Is required in the specific splicing of CDKN1A pre-mRNA; the function probbaly involves the recruitment of U2AF2 to the mRNA. Is proposed to recruit PPIL1 to the spliceosome. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA (By similarity). 
Sequence Annotation
 REGION 59 79 Interaction with PPIL1 (By similarity).
 REGION 174 339 SNW.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 224 224 Phosphoserine.
 MOD_RES 232 232 Phosphoserine.
 MOD_RES 234 234 Phosphoserine (By similarity).  
Keyword
 Acetylation; Complete proteome; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Spliceosome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 536 AA 
Protein Sequence
MALTSFLPAP TQLSQDQLEA EERARSQRSL QTSLVSSRRE PPPYGYRKGW IPRLLEDFGD 60
GGAFPEIHVA QYPLDMGRKK KMSNALAIQV DPEGKIKYDA IARQGQSKDK VIYSKYTDLV 120
PKEVMNADDP DLQRPDEEAI KEITEKTRVA LEKSVSQKVA AAMPVRAADK LAPAQYIRYT 180
PSQQGVAFNS GAKQRVIRMV EMQKEPMEPP RFKINKKIPR GPPSPPAPVM HSPSRKMTVK 240
EQQEWKIPPC ISNWKNAKGY TIPIDKRLAA DGRGLQTVHI NENFAKLAEA LYIADRKARE 300
AVEMRAQVER KMAQKEKEKH EEKLREMAQK ARERRAGIKT HVEKEDGEAR ERDEIRHDRR 360
KERQHDRNLS RAAPDKRSKL QRNENRDISE VIALGVPNPR TSNEVQYDQR LFNQSKGMDS 420
GFAGGEDEIY NVYDQAWRGG KDMAQSIYRP SKNLDKDMYG DDLEARIKTN RFVPDKEFSG 480
SDRKQRGREG PVQFEEDPFG LDKFLEEAKQ HGGSKRPSDS SRPKEHEHEG KKRRKE 536 
Gene Ontology
 GO:0016363; C:nuclear matrix; ISS:UniProtKB.
 GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
 GO:0042974; F:retinoic acid receptor binding; ISS:UniProtKB.
 GO:0046332; F:SMAD binding; ISS:UniProtKB.
 GO:0003713; F:transcription coactivator activity; ISS:UniProtKB.
 GO:0003714; F:transcription corepressor activity; ISS:UniProtKB.
 GO:0042809; F:vitamin D receptor binding; ISS:UniProtKB.
 GO:0071300; P:cellular response to retinoic acid; IDA:UniProtKB.
 GO:0042771; P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; ISS:UniProtKB.
 GO:0000398; P:mRNA splicing, via spliceosome; IEA:InterPro.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0051571; P:positive regulation of histone H3-K4 methylation; ISS:UniProtKB.
 GO:0048026; P:positive regulation of mRNA splicing, via spliceosome; ISS:UniProtKB.
 GO:0050769; P:positive regulation of neurogenesis; IDA:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0030511; P:positive regulation of transforming growth factor beta receptor signaling pathway; ISS:UniProtKB.
 GO:0048385; P:regulation of retinoic acid receptor signaling pathway; ISS:UniProtKB.
 GO:0070562; P:regulation of vitamin D receptor signaling pathway; ISS:UniProtKB.
 GO:0048384; P:retinoic acid receptor signaling pathway; ISS:UniProtKB.
 GO:0035914; P:skeletal muscle cell differentiation; IMP:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR017862; SKI-int_prot_SKIP.
 IPR004015; SKI-int_prot_SKIP_SNW-dom. 
Pfam
 PF02731; SKIP_SNW 
SMART
  
PROSITE
  
PRINTS