CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-041875
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase Mdm2 
Protein Synonyms/Alias
 Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (Mouse), isoform CRA_g 
Gene Name
 MDM2 
Gene Synonyms/Alias
 hCG_2014981 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
285ENWLPEDKGKDKGEIubiquitination[1]
287WLPEDKGKDKGEISEubiquitination[1]
289PEDKGKDKGEISEKAubiquitination[1]
295DKGEISEKAKLENSTubiquitination[1]
297GEISEKAKLENSTQAubiquitination[2]
314GFDVPDCKKTIVNDSubiquitination[2, 3, 4]
315FDVPDCKKTIVNDSRubiquitination[1, 2]
424KKLKKRNKPCPVCRQubiquitination[2, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Metal-binding; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 442 AA 
Protein Sequence
MVRSRQMCNT NMSVPTDGAV TTSQIPASEQ ETLVRPKPLL LKLLKSVGAQ KDTYTMKEVL 60
FYLGQYIMTK RLYDEKQQHI VYCSNDLLGD LFGVPSFSVK EHRKIYTMIY RNLVVVNQQE 120
ENSDELSGER QRKRHKSDSI SLSFDESLAL CVIREICCER SSSSESTGTP SNPDLDAGVS 180
EHSGDWLDQD SVSDQFSVEF EVESLDSEDY SLSEEGQELS DEDDEVYQVT VYQAGESDTD 240
SFEEDPEISL ADYWKCTSCN EMNPPLPSHC NRCWALRENW LPEDKGKDKG EISEKAKLEN 300
STQAEEGFDV PDCKKTIVND SRESCVEEND DKITQASQSQ ESEDYSQPST SSSIIYSSQE 360
DVKEFEREET QDKEESVESS LPLNAIEPCV ICQGRPKNGC IVHGKTGHLM ACFTCAKKLK 420
KRNKPCPVCR QPIQMIVLTY FP 442 
Gene Ontology
 GO:0005634; C:nucleus; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0043066; P:negative regulation of apoptotic process; IEA:InterPro.
 GO:0071157; P:negative regulation of cell cycle arrest; IEA:InterPro.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:InterPro.
 GO:0016567; P:protein ubiquitination; IEA:InterPro. 
Interpro
 IPR015459; MDM2_E3_ligase.
 IPR016495; p53_neg-reg_MDM_2/4.
 IPR003121; SWIB_MDM2_domain.
 IPR001876; Znf_RanBP2.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF02201; SWIB
 PF00641; zf-RanBP 
SMART
 SM00184; RING 
PROSITE
 PS01358; ZF_RANBP2_1
 PS50199; ZF_RANBP2_2
 PS50089; ZF_RING_2 
PRINTS