CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016188
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleolar protein 9 
Protein Synonyms/Alias
  
Gene Name
 NOP9 
Gene Synonyms/Alias
 C14orf21; KIAA2021 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
73RRALSALKEAPETGEubiquitination[1, 2]
549RRVLQNLKGQYVALAubiquitination[1, 2, 3]
578GAALRARKEIAAELGubiquitination[2]
611VALTTFLKRREAWEQubiquitination[2]
625QQQGAVAKRRRALNSubiquitination[2, 4]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
 REPEAT 92 123 Pumilio 1.
 REPEAT 189 223 Pumilio 2.
 REPEAT 313 348 Pumilio 3.
 REPEAT 351 386 Pumilio 4.
 REPEAT 509 544 Pumilio 5.
 REPEAT 547 581 Pumilio 6.
 MOD_RES 7 7 Phosphoserine.  
Keyword
 Complete proteome; Phosphoprotein; Polymorphism; Reference proteome; Repeat; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 636 AA 
Protein Sequence
MGQGPRSPHK VGRRFPAGGK RGRGAKGSGR PLPGRKRQPW PPPDGRSEPA PDSHPHLSPE 60
ALGYFRRALS ALKEAPETGE ERDLMVHNIM KEVETQALAL STNRTGSEML QELLGFSPLK 120
PLCRVWAALR SNLRTVACHR CGVHVLQSAL LQLPRLLGSA AEEEEEEEED GKDGPTETLE 180
ELVLGLAAEV CDDFLVYCGD THGSFVVRTL LQVLGGTILE SERARPRGSQ SSEAQKTPAQ 240
ECKPADFEVP ETFLNRLQDL SSSFLKDIAV FITDKISSFC LQVALQVLHR KLPQFCAHLC 300
NAVIGYLSTR GSSVDGSPLL LFLRDQTSSR LLEQVLLVLE PPRLQSLFEE HLQGQLQTLA 360
AHPIANFPLQ RLLDAVTTPE LLSPVFEELS PVLEAVLAQG HPGVVIALVG ACRRVGAYQA 420
KVLQLLLEAF HCAEPSSRQV ACVPLFATLM AYEVYYGLTE EEGAVPAEHQ VAMAAARALG 480
DVTVLGSLLL QHLLHFSTPG LVLRSLGALT GPQLLSLAQS PAGSHVLDAI LTSPSVTRKL 540
RRRVLQNLKG QYVALACSRH GSRVLDAIWS GAALRARKEI AAELGEQNQE LIRDPFGHHV 600
ARNVALTTFL KRREAWEQQQ GAVAKRRRAL NSILED 636 
Gene Ontology
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR001313; Pumilio_RNA-bd_rpt. 
Pfam
  
SMART
 SM00025; Pumilio 
PROSITE
 PS50302; PUM 
PRINTS