CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009924
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribonucleoside-diphosphate reductase 1 subunit beta 
Protein Synonyms/Alias
 Protein B2; Protein R2; Ribonucleotide reductase 1 
Gene Name
 nrdB 
Gene Synonyms/Alias
 ftsB; b2235; JW2229 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
15QTKNDQLKEPMFFGQacetylation[1]
33VARYDQQKYDIFEKLacetylation[1]
39QKYDIFEKLIEKQLSacetylation[1]
43IFEKLIEKQLSFFWRacetylation[1]
71QALPEHEKHIFISNLacetylation[1]
79HIFISNLKYQTLLDSacetylation[1]
149VTNEQIQKRAEGISSacetylation[1]
230ELMEGNAKIIRLIARacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis. 
Sequence Annotation
 ACT_SITE 123 123
 METAL 85 85 Iron 1.
 METAL 116 116 Iron 1.
 METAL 116 116 Iron 2.
 METAL 119 119 Iron 1.
 METAL 205 205 Iron 2.
 METAL 239 239 Iron 2.
 METAL 242 242 Iron 2.  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; DNA replication; Iron; Metal-binding; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 376 AA 
Protein Sequence
MAYTTFSQTK NDQLKEPMFF GQPVNVARYD QQKYDIFEKL IEKQLSFFWR PEEVDVSRDR 60
IDYQALPEHE KHIFISNLKY QTLLDSIQGR SPNVALLPLI SIPELETWVE TWAFSETIHS 120
RSYTHIIRNI VNDPSVVFDD IVTNEQIQKR AEGISSYYDE LIEMTSYWHL LGEGTHTVNG 180
KTVTVSLREL KKKLYLCLMS VNALEAIRFY VSFACSFAFA ERELMEGNAK IIRLIARDEA 240
LHLTGTQHML NLLRSGADDP EMAEIAEECK QECYDLFVQA AQQEKDWADY LFRDGSMIGL 300
NKDILCQYVE YITNIRMQAV GLDLPFQTRS NPIPWINTWL VSDNVQVAPQ EVEVSSYLVG 360
QIDSEVDTDD LSNFQL 376 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005971; C:ribonucleoside-diphosphate reductase complex; IDA:EcoliWiki.
 GO:0005506; F:iron ion binding; IDA:EcoCyc.
 GO:0004748; F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor; IEA:EC.
 GO:0009186; P:deoxyribonucleoside diphosphate metabolic process; IEA:InterPro.
 GO:0009263; P:deoxyribonucleotide biosynthetic process; IDA:EcoliWiki.
 GO:0006260; P:DNA replication; IEA:UniProtKB-UniPathway.
 GO:0015949; P:nucleobase-containing small molecule interconversion; IDA:EcoliWiki. 
Interpro
 IPR009078; Ferritin-like_SF.
 IPR012348; RNR-rel.
 IPR000358; RNR_small. 
Pfam
 PF00268; Ribonuc_red_sm 
SMART
  
PROSITE
 PS00368; RIBORED_SMALL 
PRINTS