UniProt Accession

 CNOT8_MOUSE; Q9D8X5; Q3U532 

Genbank Protein ID

 AK007581; AK075727; AK150757; AK153907; BC004040 

Genbank Nucleotide ID

 BAB25119.1; BAC35913.1; BAE29826.1; BAE32248.1; AAH04040.1 

Protein Name

 CCR4-NOT transcription complex subunit 8 

Protein Synonyms/Alias

 CCR4-associated factor 8 

Gene Name

 Cnot8 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
206	MKSCKNLKGGLQEVA	ubiquitination	[1]
254	EDSIDDAKYCGRLYG	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT7. Catalytic component of the CCR4-NOT complex which is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity. 

Sequence Annotation

 METAL 40 40 Divalent metal cation 1; catalytic (By
 METAL 40 40 Divalent metal cation 2; catalytic (By
 METAL 42 42 Divalent metal cation 2; catalytic (By
 METAL 161 161 Divalent metal cation 1; catalytic (By
 METAL 230 230 Divalent metal cation 2; catalytic (By  

Keyword

 Complete proteome; Cytoplasm; Exonuclease; Hydrolase; Metal-binding; Nuclease; Nucleus; Reference proteome; Repressor; RNA-binding; RNA-mediated gene silencing; Transcription; Transcription regulation; Translation regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 292 AA 

Protein Sequence

Gene Ontology

 GO:0030014; C:CCR4-NOT complex; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004535; F:poly(A)-specific ribonuclease activity; ISS:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0043928; P:exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay; ISS:UniProtKB.
 GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
 GO:0008284; P:positive regulation of cell proliferation; ISS:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR027212; Cnot8.
 IPR006941; RNase_CAF1.
 IPR012337; RNaseH-like_dom. 

Pfam

 PF04857; CAF1 

SMART

  

PROSITE

  

PRINTS