UniProt Accession

 6PGD1_YEAST; P38720; D3DLD1 

Genbank Protein ID

 Z46631; U00028; U17155; BK006934 

Genbank Nucleotide ID

 CAA86600.1; AAB68452.1; AAA53637.1; DAA06875.1 

Protein Name

 6-phosphogluconate dehydrogenase, decarboxylating 1 

Protein Synonyms/Alias

  

Gene Name

 GND1 

Gene Synonyms/Alias

 YHR183W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
37	AYNRTQSKVDHFLAN	acetylation	[1]
37	AYNRTQSKVDHFLAN	ubiquitination	[2]
47	HFLANEAKGKSIIGA	acetylation	[1]
75	RKVMLLVKAGAPVDA	ubiquitination	[3]
212	RLGGFTDKEISDVFA	acetylation	[1]
239	EITRDILKFDDVDGK	acetylation	[1]
246	KFDDVDGKPLVEKIM	acetylation	[1]
246	KFDDVDGKPLVEKIM	ubiquitination	[2]
259	IMDTAGQKGTGKWTA	ubiquitination	[2]
263	AGQKGTGKWTAINAL	ubiquitination	[2]
292	ARCLSALKNERIRAS	ubiquitination	[2]
309	LPGPEVPKDAVKDRE	acetylation	[1]
313	EVPKDAVKDREQFVD	acetylation	[1]
377	VFLGQITKAYREEPD	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 Catalyzes the oxidative decarboxylation of 6- phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH. 

Sequence Annotation

 NP_BIND 9 14 NADP (By similarity).
 NP_BIND 32 34 NADP (By similarity).
 NP_BIND 74 76 NADP (By similarity).
 REGION 128 130 Substrate binding (By similarity).
 REGION 185 186 Substrate binding (By similarity).
 ACT_SITE 182 182 Proton acceptor (By similarity).
 ACT_SITE 189 189 Proton donor (By similarity).
 BINDING 102 102 NADP (By similarity).
 BINDING 102 102 Substrate (By similarity).
 BINDING 190 190 Substrate (By similarity).
 BINDING 259 259 Substrate; via amide nitrogen (By
 BINDING 286 286 Substrate (By similarity).
 BINDING 446 446 Substrate; shared with dimeric partner
 BINDING 452 452 Substrate; shared with dimeric partner
 MOD_RES 50 50 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Gluconate utilization; NADP; Oxidoreductase; Pentose shunt; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 489 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0004616; F:phosphogluconate dehydrogenase (decarboxylating) activity; IMP:SGD.
 GO:0034599; P:cellular response to oxidative stress; IMP:SGD.
 GO:0019521; P:D-gluconate metabolic process; IEA:UniProtKB-KW.
 GO:0009051; P:pentose-phosphate shunt, oxidative branch; IMP:SGD. 

Interpro

 IPR008927; 6-PGluconate_DH_C-like.
 IPR006114; 6PGDH_C.
 IPR006113; 6PGDH_decarbox.
 IPR006115; 6PGDH_NADP-bd.
 IPR006184; 6PGdom_BS.
 IPR013328; DH_multihelical.
 IPR012284; Fibritin/6PGD_C-extension.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF00393; 6PGD
 PF03446; NAD_binding_2 

SMART

  

PROSITE

 PS00461; 6PGD 

PRINTS