CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024315
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cell division cycle protein 27 homolog 
Protein Synonyms/Alias
  
Gene Name
 Cdc27 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
62YKAYRLLKGHSCTTPubiquitination[1]
565KDLTDMDKNSPEAWCubiquitination[1]
589REHDIAIKFFQRAIQubiquitination[1, 2, 3]
688HALKKSEKALDTLNKubiquitination[1]
695KALDTLNKAIVIDPKubiquitination[1, 3]
702KAIVIDPKNPLCKFHubiquitination[1]
707DPKNPLCKFHRASVLubiquitination[1]
719SVLFANEKYKSALQEubiquitination[1]
721LFANEKYKSALQELEubiquitination[1]
731LQELEELKQIVPKESubiquitination[1]
777KGANNQIKEAIDKRYubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity). 
Sequence Annotation
 REPEAT 84 114 TPR 1.
 REPEAT 115 148 TPR 2.
 REPEAT 500 533 TPR 3.
 REPEAT 568 601 TPR 4.
 REPEAT 603 635 TPR 5.
 REPEAT 636 669 TPR 6.
 REPEAT 671 703 TPR 7.
 REPEAT 705 737 TPR 8.
 REPEAT 738 771 TPR 9.
 MOD_RES 205 205 Phosphothreonine (By similarity).
 MOD_RES 209 209 Phosphothreonine (By similarity).
 MOD_RES 291 291 Phosphoserine (By similarity).
 MOD_RES 313 313 Phosphothreonine (By similarity).
 MOD_RES 339 339 Phosphoserine (By similarity).
 MOD_RES 367 367 Phosphothreonine (By similarity).
 MOD_RES 387 387 Phosphoserine (By similarity).
 MOD_RES 427 427 Phosphoserine (By similarity).
 MOD_RES 431 431 Phosphothreonine (By similarity).
 MOD_RES 436 436 Phosphoserine (By similarity).
 MOD_RES 439 439 Phosphoserine (By similarity).
 MOD_RES 447 447 Phosphothreonine (By similarity).
 MOD_RES 822 822 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat; TPR repeat; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 825 AA 
Protein Sequence
MTVLQEPVQA AIWQALNHYA YRDAVFLAER LYAEVHSEEA LFLLATCYYR SGKAYKAYRL 60
LKGHSCTTPQ CKYLLAKCCV DLSKLAEGEQ ILSGGVFNKQ KSHDDLVTEF GDSACFTLSL 120
LGHVYCKTDR LAKGSECYQK SLSLNPFLWS PFESLCEIGE KPDPDQTFKL TSLQNFSSCL 180
PNTCTTLVSN HSLSHRQPET VLTETPQDTI ELNRLNLESS NSKYSLNTDS SVSYIDSTVI 240
SPDNVPLGPG TAILSKQVQN KPKTGRSLLG GPTALSPLTP SFGILPLETP SPGDGSYLQN 300
YTNTPSVIDV APTGAPTKKS VARMGQTGTK SVFSQSGNSR EVTPVLVAQT QSSGPQTSTT 360
PQVLSPTITS PPNALPRRSS RLFTSDSSTT KENSKKLKMK FPPKIPNRKT KSKTNKGGLT 420
QPSINDSLEI TKLDSSIISE GKITTVTPQI QAFNLQKAAA EGLMSLLREM GKGYLALCSY 480
NCKEAINILS HLPSHHYSTG WVLCQIGRAY FELSEYMQAE RIFSEVRRIE SFRVEGMEIY 540
STTLWHLQKD VALSVLSKDL TDMDKNSPEA WCAAGNCFSL QREHDIAIKF FQRAIQVDPN 600
YAYAYTLLGH EFVLTEELDK ALACFRNAIR VNPRHYNAWY GLGMIYYKQE KFSLAEMHFQ 660
KALDINPQSS VLLCHIGVVQ HALKKSEKAL DTLNKAIVID PKNPLCKFHR ASVLFANEKY 720
KSALQELEEL KQIVPKESLV YFLIGKVYKK LGQTHLALMN FSWAMDLDPK GANNQIKEAI 780
DKRYLPDDEE PITQEEQIMG TDESQESSMT DADDTQLHAA ESDEF 825 
Gene Ontology
 GO:0005680; C:anaphase-promoting complex; ISS:UniProtKB.
 GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; IEA:InterPro.
 GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB. 
Interpro
 IPR026803; CDC27.
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR013105; TPR_2.
 IPR019734; TPR_repeat. 
Pfam
 PF00515; TPR_1
 PF07719; TPR_2 
SMART
 SM00028; TPR 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS