CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021758
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Interleukin enhancer-binding factor 3 
Protein Synonyms/Alias
  
Gene Name
 Ilf3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
100MRVGLVAKGLLLKGDubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can promote the formation of stable DNA- dependent protein kinase holoenzyme complexes on DNA (By similarity). Can regulate protein arginine N-methyltransferase 1 activity. The phosphorylated form at Thr-188 and Thr-315, in concert with EIF2AK2/PKR can inhibit vesicular stomatitis virus (VSV) replication (By similarity). 
Sequence Annotation
 DOMAIN 94 342 DZF.
 DOMAIN 398 467 DRBM 1.
 DOMAIN 524 590 DRBM 2.
 REGION 609 897 Interaction with PRMT1.
 MOTIF 371 389 Bipartite nuclear localization signal
 MOD_RES 100 100 N6-acetyllysine (By similarity).
 MOD_RES 188 188 Phosphothreonine; by PKR (By similarity).
 MOD_RES 190 190 Phosphoserine (By similarity).
 MOD_RES 315 315 Phosphothreonine; by PKR (By similarity).
 MOD_RES 382 382 Phosphoserine (By similarity).
 MOD_RES 384 384 Phosphoserine (By similarity).
 MOD_RES 460 460 N6-acetyllysine (By similarity).
 MOD_RES 476 476 Phosphoserine (By similarity).
 MOD_RES 482 482 Phosphoserine (By similarity).
 MOD_RES 609 609 Omega-N-methylated arginine (By
 MOD_RES 794 794 Phosphoserine (By similarity).
 MOD_RES 814 814 Phosphoserine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Antiviral defense; Complete proteome; Cytoplasm; DNA-binding; Methylation; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 897 AA 
Protein Sequence
MRPMRIFVND DRHVMAKHSS VYPTQEELEA VQNMVSHTER ALKAVSDWID EQEKGNSELS 60
EAENMDTPPD DESKEGAGEQ KAEHMTRTLR GVMRVGLVAK GLLLKGDLDL ELVLLCKEKP 120
TTALLDKVAD NLAIQLTTVT EDKYEILQSV DDAAIVIKNT KEPPLSLTIH LTSPVVREEM 180
EKVLAGETLS VNDPPDVLDR QKCLAALASL RHAKWFQARA NGLKSCVIVI RVLRDLCTRV 240
PTWGPLRGWP LELLCEKSIG TANRPMGAGE ALRRVLECLA SGIVMPDGSG IYDPCEKEAT 300
DAIGHLDRQQ REDITQSAQH ALRLAAFGQL HKVLGMDPLP SKMPKKPKNE NPVDYTVQIP 360
PSTTYAITPM KRPMEEDGEE KSPSKKKKKI QKKEEKAEPP QAMNALMRLN QLKPGLQYKL 420
ISQTGPVHAP IFTMSVEVDG STFEASGPSK KTAKLHVAVK VLQDMGLPTG AEGRDSSKGE 480
DSAEESDGKP AVVAPPPVVE AVSNPSSVFP SDATTEQGPI LTKHGKNPVM ELNEKRRGLK 540
YELISETGGS HDKRFVMEVE VDGQKFQGAG SNKKVAKAYA ALAALEKLFP DAPLALEANK 600
KKRAPVPVRG GPKFAAKPHN PGFGMGGPMH NEAPPPPNIR GRGRGGNIRG RGRGRGFGGT 660
NHGGGYMNAG AGYGSYGYSS NSATAGYSQF YSNGGHYGNA GGGGSGGGGG SSSYSSYYQG 720
DSYNSPVPPK HAGKKPLHGG QQKPSYSSGY QSHQGQQQPY NQSQYSSYGT PQGKQKGYGH 780
GQGSYSSYSN SYNSPGGGGG SDYSYDSKFN YSGSGGRSGG NSYGSSGSSY NTGSHGGYGA 840
GSGGSSSYQG KQGGYSSQSN YSSPGSSQSY SGPASSYQSS QGGYSRNTEH SMNYQYR 897 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0030529; C:ribonucleoprotein complex; ISS:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:RGD.
 GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
 GO:0017148; P:negative regulation of translation; ISS:UniProtKB.
 GO:0045071; P:negative regulation of viral genome replication; ISS:UniProtKB.
 GO:0006479; P:protein methylation; IDA:MGI.
 GO:0006468; P:protein phosphorylation; ISS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001159; Ds-RNA-bd.
 IPR014720; dsRNA-bd-like_dom.
 IPR006561; DZF. 
Pfam
 PF00035; dsrm
 PF07528; DZF 
SMART
 SM00358; DSRM
 SM00572; DZF 
PROSITE
 PS50137; DS_RBD 
PRINTS