CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022411
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 G2 and S phase-expressed protein 1 
Protein Synonyms/Alias
 GTSE-1; Protein B99 homolog 
Gene Name
 GTSE1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
106NQAAQAAKPEDPRSQubiquitination[1, 2]
123ERFIQESKLKINLFEubiquitination[1]
125FIQESKLKINLFEKEubiquitination[1, 2]
133INLFEKEKEMKKSPTubiquitination[1]
455TNQFKIPKFSIGDSPubiquitination[3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 May be involved in p53-induced cell cycle arrest in G2/M phase by interfering with microtubule rearrangements that are required to enter mitosis. Overexpression delays G2/M phase progression. 
Sequence Annotation
 MOD_RES 72 72 Phosphoserine.
 MOD_RES 138 138 Phosphoserine.
 MOD_RES 140 140 Phosphothreonine.
 MOD_RES 152 152 Phosphoserine.
 MOD_RES 168 168 Phosphoserine.
 MOD_RES 189 189 Phosphoserine.
 MOD_RES 477 477 Phosphoserine.
 MOD_RES 480 480 Phosphoserine.
 MOD_RES 495 495 Phosphoserine.
 MOD_RES 501 501 Phosphoserine.
 MOD_RES 504 504 Phosphoserine.
 MOD_RES 509 509 Phosphoserine.
 MOD_RES 513 513 Phosphothreonine.
 MOD_RES 516 516 Phosphoserine.
 MOD_RES 536 536 Phosphoserine.
 MOD_RES 575 575 Phosphoserine.
 MOD_RES 592 592 Phosphoserine.
 MOD_RES 677 677 Phosphothreonine.
 MOD_RES 688 688 Phosphoserine.
 MOD_RES 698 698 Phosphoserine.
 MOD_RES 699 699 Phosphoserine.
 MOD_RES 705 705 Phosphoserine.
 MOD_RES 715 715 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 720 AA 
Protein Sequence
MDDPKKEDIL LLADEKFDFD LSLSSSSANE DDEVFFGPFG HKERCIAASL ELNNPVPEQP 60
PLPTSESPFA WSPLAGEKFV EVYKEAHLLA LHIESSSRNQ AAQAAKPEDP RSQGVERFIQ 120
ESKLKINLFE KEKEMKKSPT SLKRETYYLS DSPLLGPPVG EPRLLASSPA LPSSGAQARL 180
TRAPGPPHSA HALPRESCTA HAASQAATQR KPGTKLLLPR AASVRGRSIP GAAEKPKKEI 240
PASPSRTKIP AEKESHRDVL PDKPAPGAVN VPAAGSHLGQ GKRAIPVPNK LGLKKTLLKA 300
PGSTSNLARK SSSGPVWSGA SSACTSPAVG KAKSSEFASI PANSSRPLSN ISKSGRMGPA 360
MLRPALPAGP VGASSWQAKR VDVSELAAEQ LTAPPSASPT QPQTPEGGGQ WLNSSCAWSE 420
SSQLNKTRSI RRRDSCLNSK TKVMPTPTNQ FKIPKFSIGD SPDSSTPKLS RAQRPQSCTS 480
VGRVTVHSTP VRRSSGPAPQ SLLSAWRVSA LPTPASRRCS GLPPMTPKTM PRAVGSPLCV 540
PARRRSSEPR KNSAMRTEPT RESNRKTDSR LVDVSPDRGS PPSRVPQALN FSPEESDSTF 600
SKSTATEVAR EEAKPGGDAA PSEALLVDIK LEPLAVTPDA ASQPLIDLPL IDFCDTPEAH 660
VAVGSESRPL IDLMTNTPDM NKNVAKPSPV VGQLIDLSSP LIQLSPEADK ENVDSPLLKF 720 
Gene Ontology
 GO:0005881; C:cytoplasmic microtubule; NAS:UniProtKB.
 GO:0006977; P:DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; NAS:UniProtKB.
 GO:0000085; P:G2 phase of mitotic cell cycle; NAS:UniProtKB.
 GO:0007017; P:microtubule-based process; NAS:UniProtKB. 
Interpro
 IPR026657; DDA3/GTSE-1.
 IPR026659; GTSE1. 
Pfam
  
SMART
  
PROSITE
  
PRINTS