CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017048
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cell division cycle and apoptosis regulator protein 1 
Protein Synonyms/Alias
 Cell cycle and apoptosis regulatory protein 1; CARP-1; Death inducer with SAP domain 
Gene Name
 CCAR1 
Gene Synonyms/Alias
 CARP1; DIS 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
368RYTVQFSKFSLDCPSubiquitination[1]
486QHPARLVKFLVGMKGubiquitination[2, 3]
594ETLSRGYKQQLVEKLubiquitination[1]
637HWSKLDPKTMKVNDLubiquitination[3, 4]
1012LLPTPTVKQESKDVEsumoylation[5]
1054VRAEVEQKLQLLEEKubiquitination[2, 3]
1088GELREVKKDLSQLQEubiquitination[2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells.
 Tan F, Lu L, Cai Y, Wang J, Xie Y, Wang L, Gong Y, Xu BE, Wu J, Luo Y, Qiang B, Yuan J, Sun X, Peng X.
 Proteomics. 2008 Jul;8(14):2885-96. [PMID: 18655026]
 [5] Site-specific identification of SUMO-2 targets in cells reveals an inverted SUMOylation motif and a hydrophobic cluster SUMOylation motif.
 Matic I, Schimmel J, Hendriks IA, van Santen MA, van de Rijke F, van Dam H, Gnad F, Mann M, Vertegaal AC.
 Mol Cell. 2010 Aug 27;39(4):641-52. [PMID: 20797634] 
Functional Description
 Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation. 
Sequence Annotation
 DOMAIN 636 670 SAP.
 MOD_RES 685 685 Phosphoserine.
 MOD_RES 697 697 Phosphoserine.
 MOD_RES 861 861 Phosphothreonine.
 CROSSLNK 637 637 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative splicing; Apoptosis; Cell cycle; Coiled coil; Complete proteome; Cytoplasm; Isopeptide bond; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1150 AA 
Protein Sequence
MAQFGGQKNP PWATQFTATA VSQPAALGVQ QPSLLGASPT IYTQQTALAA AGLTTQTPAN 60
YQLTQTAALQ QQAAAAAAAL QQQYSQPQQA LYSVQQQLQQ PQQTLLTQPA VALPTSLSLS 120
TPQPTAQITV SYPTPRSSQQ QTQPQKQRVF TGVVTKLHDT FGFVDEDVFF QLSAVKGKTP 180
QVGDRVLVEA TYNPNMPFKW NAQRIQTLPN QNQSQTQPLL KTPPAVLQPI APQTTFGVQT 240
QPQPQSLLQA QISAASITPL LQTQPQPLLQ QPQQKAGLLQ PPVRIVSQPQ PARRLDPPSR 300
FSGRNDRGDQ VPNRKDDRSR ERERERRRSR ERSPQRKRSR ERSPRRERER SPRRVRRVVP 360
RYTVQFSKFS LDCPSCDMME LRRRYQNLYI PSDFFDAQFT WVDAFPLSRP FQLGNYCNFY 420
VMHREVESLE KNMAILDPPD ADHLYSAKVM LMASPSMEDL YHKSCALAED PQELRDGFQH 480
PARLVKFLVG MKGKDEAMAI GGHWSPSLDG PDPEKDPSVL IKTAIRCCKA LTGIDLSVCT 540
QWYRFAEIRY HRPEETHKGR TVPAHVETVV LFFPDVWHCL PTRSEWETLS RGYKQQLVEK 600
LQGERKEADG EQDEEEKDDG EAKEISTPTH WSKLDPKTMK VNDLRKELES RALSSKGLKS 660
QLIARLTKQL KVEEQKEEQK ELEKSEKEED EDDDRKSEDD KEEEERKRQE EIERQRRERR 720
YILPDEPAII VHPNWAAKSG KFDCSIMSLS VLLDYRLEDN KEHSFEVSLF AELFNEMLQR 780
DFGVRIYKSL LSLPEKEDKK EKDKKSKKDE RKDKKEERDD ETDEPKPKRR KSGDDKDKKE 840
DRDERKKEDK RKDDSKDDDE TEEDNNQDEY DPMEAEEAED EEDDRDEEEM TKRDDKRDIN 900
RYCKERPSKD KEKEKTQMIT INRDLLMAFV YFDQSHCGYL LEKDLEEILY TLGLHLSRAQ 960
VKKLLNKVVL RESCFYRKLT DTSKDEENHE ESESLQEDML GNRLLLPTPT VKQESKDVEE 1020
NVGLIVYNGA MVDVGSLLQK LEKSEKVRAE VEQKLQLLEE KTDEDEKTIL NLENSNKSLS 1080
GELREVKKDL SQLQENLKIS ENMNLQFENQ MNKTIRNLST VMDEIHTVLK KDNVKNEDKD 1140
QKSKENGASV 1150 
Gene Ontology
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0030374; F:ligand-dependent nuclear receptor transcription coactivator activity; IGI:MGI.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR025224; DBC1/CARP1.
 IPR025954; DBC1/CARP1_inactive_NUDIX_dom.
 IPR012340; NA-bd_OB-fold.
 IPR025223; S1-like_RNA-bd_dom.
 IPR003034; SAP_dom. 
Pfam
 PF14443; DBC1
 PF14444; S1-like
 PF02037; SAP 
SMART
 SM00513; SAP 
PROSITE
 PS50800; SAP 
PRINTS