UniProt Accession

 PUR6_HUMAN; P22234; E9PDH9; Q68CQ5 

Genbank Protein ID

 X53793; BT006988; CR749824; AC068620; AC114766; BC010273; BC019255 

Genbank Nucleotide ID

 CAA37801.1; AAP35634.1; CAH18683.1; AAH10273.1; AAH19255.1 

Protein Name

 Multifunctional protein ADE2 

Protein Synonyms/Alias

 Phosphoribosylaminoimidazole-succinocarboxamide synthase; SAICAR synthetase; Phosphoribosylaminoimidazole carboxylase; AIR carboxylase; AIRC 

Gene Name

 PAICS 

Gene Synonyms/Alias

 ADE2; AIRC; PAIS 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
11	AEVLNIGKKLYEGKT	ubiquitination	[1]
19	KLYEGKTKEVYELLD	ubiquitination	[1]
30	ELLDSPGKVLLQSKD	ubiquitination	[1]
36	GKVLLQSKDQITAGN	ubiquitination	[1, 2]
117	IATGSFLKRNPGVKE	ubiquitination	[1]
138	PKVELFFKDDANNDP	ubiquitination	[2]
242	KQSYRDLKEVTPEGL	ubiquitination	[2]
253	PEGLQMVKKNFEWVA	ubiquitination	[2]
311	LRVTSAHKGPDETLR	ubiquitination	[1, 2]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

  

Sequence Annotation

 REGION 2 260 SAICAR synthetase.
 REGION 261 266 Linker.
 REGION 267 425 AIR carboxylase.
 ACT_SITE 101 101 For SAICAR synthetase activity.
 ACT_SITE 107 107 For SAICAR synthetase activity.
 ACT_SITE 215 215 For SAICAR synthetase activity.
 ACT_SITE 303 303 For AIR carboxylase activity.
 ACT_SITE 332 332 For AIR carboxylase activity.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 22 22 Phosphotyrosine (By similarity).
 MOD_RES 27 27 Phosphoserine.
 MOD_RES 107 107 Phosphoserine.
 MOD_RES 238 238 Phosphothreonine.
 MOD_RES 247 247 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Decarboxylase; Direct protein sequencing; Ligase; Lyase; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Polymorphism; Purine biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 425 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0034023; F:5-(carboxyamino)imidazole ribonucleotide mutase activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004638; F:phosphoribosylaminoimidazole carboxylase activity; TAS:Reactome.
 GO:0004639; F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; TAS:Reactome.
 GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0009113; P:purine nucleobase biosynthetic process; TAS:ProtInc.
 GO:0009168; P:purine ribonucleoside monophosphate biosynthetic process; TAS:Reactome. 

Interpro

 IPR013816; ATP_grasp_subdomain_2.
 IPR000031; N5-CAIR_Mutase_PurE_dom.
 IPR001636; SAICAR_synth.
 IPR018236; SAICAR_synthetase_CS. 

Pfam

 PF00731; AIRC
 PF01259; SAICAR_synt 

SMART

 SM01001; AIRC 

PROSITE

 PS01057; SAICAR_SYNTHETASE_1
 PS01058; SAICAR_SYNTHETASE_2 

PRINTS