CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016218
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 48 
Protein Synonyms/Alias
 Deubiquitinating enzyme 48; Ubiquitin thioesterase 48; Ubiquitin-specific-processing protease 48 
Gene Name
 USP48 
Gene Synonyms/Alias
 USP31 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
90GGPRLTVKALCKECVubiquitination[1, 2]
94LTVKALCKECVVERCubiquitination[2]
126NLLKAAVKGDGFWVGubiquitination[2]
207NERRLVSKEAWSKLQubiquitination[1, 2]
212VSKEAWSKLQQYFPKubiquitination[2]
219KLQQYFPKAPEFPSYubiquitination[1]
227APEFPSYKECCSQCKubiquitination[2]
234KECCSQCKILEREGEubiquitination[2]
406QATIYVHKVVDNKKVubiquitination[2]
412HKVVDNKKVMKDSAPubiquitination[2]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Recognizes and hydrolyzes the peptide bond at the C- terminal Gly of ubiquitin. Involved in the processing of poly- ubiquitin precursors as well as that of ubiquitinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites. 
Sequence Annotation
 DOMAIN 460 554 DUSP 1.
 DOMAIN 569 691 DUSP 2.
 DOMAIN 711 824 DUSP 3.
 DOMAIN 929 1009 Ubiquitin-like.
 ACT_SITE 98 98 Nucleophile (By similarity).
 ACT_SITE 353 353 Proton acceptor (By similarity).
 MOD_RES 886 886 Phosphoserine.
 MOD_RES 887 887 Phosphoserine.
 MOD_RES 888 888 Phosphoserine.
 MOD_RES 956 956 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Hydrolase; Nucleus; Phosphoprotein; Polymorphism; Protease; Reference proteome; Repeat; Thiol protease; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1035 AA 
Protein Sequence
MNLSLSQKTV KIHRLFPMLA FSEPYEFVSL EWLQKWLDES TPTKPIDNHA CLCSHDKLHP 60
DKISIMKRIS EYAADIFYSR YGGGPRLTVK ALCKECVVER CRILRLKNQL NEDYKTVNNL 120
LKAAVKGDGF WVGKSSLRSW RQLALEQLDE QDGDAEQSNG KMNGSTLNKD ESKEERKEEE 180
ELNFNEDILC PHGELCISEN ERRLVSKEAW SKLQQYFPKA PEFPSYKECC SQCKILEREG 240
EENEALHKMI ANEQKTSLPN LFQDKNRPCL SNWPEDTDVL YIVSQFFVEE WRKFVRKPTR 300
CSPVSSVGNS ALLCPHGGLM FTFASMTKED SKLIALIWPS EWQMIQKLFV VDHVIKITRI 360
EVGDVNPSET QYISEPKLCP ECREGLLCQQ QRDLREYTQA TIYVHKVVDN KKVMKDSAPE 420
LNVSSSETEE DKEEAKPDGE KDPDFNQIMH AFSVAPFDQN LSIDGKILSD DCATLGTLGV 480
IPESVILLKA DEPIADYAAM DDVMQVCMPE EGFKGTGLLG H 521 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR006615; Pept_C19_DUSP.
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19.
 IPR019955; Ubiquitin_supergroup. 
Pfam
 PF00443; UCH 
SMART
  
PROSITE
 PS51283; DUSP
 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 
PRINTS