CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005573
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Soluble pyridine nucleotide transhydrogenase 
Protein Synonyms/Alias
 STH; NAD(P)(+) transhydrogenase [B-specific] 
Gene Name
 sthA 
Gene Synonyms/Alias
 sth; udhA; b3962; JW5551 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
54HWGTIPSKALRHAVSacetylation[1]
201IFRGMDVKVDLINTRacetylation[1]
261LKSGKKLKADCLLYAacetylation[1]
370EQQLTAMKVPYEVGRacetylation[1]
381EVGRAQFKHLARAQIacetylation[1, 2]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 
Functional Description
 Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation. 
Sequence Annotation
 NP_BIND 36 45 FAD (By similarity).  
Keyword
 Complete proteome; Cytoplasm; Direct protein sequencing; FAD; Flavoprotein; NAD; NADP; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 466 AA 
Protein Sequence
MPHSYDYDAI VIGSGPGGEG AAMGLVKQGA RVAVIERYQN VGGGCTHWGT IPSKALRHAV 60
SRIIEFNQNP LYSDHSRLLR SSFADILNHA DNVINQQTRM RQGFYERNHC EILQGNARFV 120
DEHTLALDCP DGSVETLTAE KFVIACGSRP YHPTDVDFTH PRIYDSDSIL SMHHEPRHVL 180
IYGAGVIGCE YASIFRGMDV KVDLINTRDR LLAFLDQEMS DSLSYHFWNS GVVIRHNEEY 240
EKIEGCDDGV IMHLKSGKKL KADCLLYANG RTGNTDSLAL QNIGLETDSR GQLKVNSMYQ 300
TAQPHVYAVG DVIGYPSLAS AAYDQGRIAA QALVKGEATA HLIEDIPTGI YTIPEISSVG 360
KTEQQLTAMK VPYEVGRAQF KHLARAQIVG MNVGTLKILF HRETKEILGI HCFGERAAEI 420
IHIGQAIMEQ KGGGNTIEYF VNTTFNYPTM AEAYRVAALN GLNRLF 466 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0003957; F:NAD(P)+ transhydrogenase (B-specific) activity; IDA:EcoCyc.
 GO:0045454; P:cell redox homeostasis; IMP:EcoCyc.
 GO:0006739; P:NADP metabolic process; IEA:HAMAP. 
Interpro
 IPR016156; FAD/NAD-linked_Rdtase_dimer.
 IPR013027; FAD_pyr_nucl-diS_OxRdtase.
 IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
 IPR023753; Pyr_nucl-diS_OxRdtase_FAD/NAD.
 IPR001327; Pyr_OxRdtase_NAD-bd_dom.
 IPR022962; STH. 
Pfam
 PF00070; Pyr_redox
 PF07992; Pyr_redox_2
 PF02852; Pyr_redox_dim 
SMART
  
PROSITE
  
PRINTS
 PR00368; FADPNR.