CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000456
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Multidrug resistance-associated protein 4 
Protein Synonyms/Alias
 ATP-binding cassette sub-family C member 4; MRP/cMOAT-related ABC transporter; Multi-specific organic anion transporter B; MOAT-B 
Gene Name
 ABCC4 
Gene Synonyms/Alias
 MRP4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
66ELQGFWDKEVLRAENubiquitination[1, 2, 3]
77RAENDAQKPSLTRAIubiquitination[1, 2, 3]
264LFSSLRSKTATFTDAubiquitination[1, 2]
293IKMYAWEKSFSNLITubiquitination[1]
304NLITNLRKKEISKILubiquitination[1]
309LRKKEISKILRSSCLubiquitination[2]
515IKACALKKDLQLLEDubiquitination[1, 3]
540TTLSGGQKARVNLARubiquitination[1]
607ASQILILKDGKMVQKubiquitination[1]
614KDGKMVQKGTYTEFLubiquitination[1]
622GTYTEFLKSGIDFGSubiquitination[1, 3, 4]
632IDFGSLLKKDNEESEubiquitination[1, 3]
633DFGSLLKKDNEESEQubiquitination[1, 3]
670QSSRPSLKDGALESQubiquitination[1, 3]
695EENRSEGKVGFQAYKubiquitination[1]
702KVGFQAYKNYFRAGAubiquitination[1, 5, 6]
807KMFESILKAPVLFFDubiquitination[3]
918LWTIRAYKAEERCQEubiquitination[1]
1021IEYTDLEKEAPWEYQubiquitination[1, 2, 3]
1070ALIKSQEKVGIVGRTubiquitination[1]
1165TIEDLPGKMDTELAEubiquitination[1]
1253VLDSGRLKEYDEPYVubiquitination[1, 3]
1265PYVLLQNKESLFYKMubiquitination[1, 3, 5, 6, 7]
1271NKESLFYKMVQQLGKubiquitination[3, 5, 6]
1278KMVQQLGKAEAAALTubiquitination[1, 3, 4, 7]
1289AALTETAKQVYFKRNubiquitination[5, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 May be an organic anion pump relevant to cellular detoxification. 
Sequence Annotation
 DOMAIN 92 377 ABC transmembrane type-1 1.
 DOMAIN 410 633 ABC transporter 1.
 DOMAIN 714 1005 ABC transmembrane type-1 2.
 DOMAIN 1041 1274 ABC transporter 2.
 NP_BIND 445 452 ATP 1 (Potential).
 NP_BIND 1075 1082 ATP 2 (Potential).
 MOTIF 1322 1325 PDZ-binding.
 MOD_RES 646 646 Phosphothreonine.
 CARBOHYD 651 651 N-linked (GlcNAc...) (Potential).
 CARBOHYD 690 690 N-linked (GlcNAc...) (Potential).
 CARBOHYD 746 746 N-linked (GlcNAc...) (Potential).
 CARBOHYD 754 754 N-linked (GlcNAc...) (Potential).
 CARBOHYD 792 792 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1176 1176 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1309 1309 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Glycoprotein; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1325 AA 
Protein Sequence
MLPVYQEVKP NPLQDANLCS RVFFWWLNPL FKIGHKRRLE EDDMYSVLPE DRSQHLGEEL 60
QGFWDKEVLR AENDAQKPSL TRAIIKCYWK SYLVLGIFTL IEESAKVIQP IFLGKIINYF 120
ENYDPMDSVA LNTAYAYATV LTFCTLILAI LHHLYFYHVQ CAGMRLRVAM CHMIYRKALR 180
LSNMAMGKTT TGQIVNLLSN DVNKFDQVTV FLHFLWAGPL QAIAVTALLW MEIGISCLAG 240
MAVLIILLPL QSCFGKLFSS LRSKTATFTD ARIRTMNEVI TGIRIIKMYA WEKSFSNLIT 300
NLRKKEISKI LRSSCLRGMN LASFFSASKI IVFVTFTTYV LLGSVITASR VFVAVTLYGA 360
VRLTVTLFFP SAIERVSEAI VSIRRIQTFL LLDEISQRNR QLPSDGKKMV HVQDFTAFWD 420
KASETPTLQG LSFTVRPGEL LAVVGPVGAG KSSLLSAVLG ELAPSHGLVS VHGRIAYVSQ 480
QPWVFSGTLR SNILFGKKYE KERYEKVIKA CALKKDLQLL EDGDLTVIGD RGTTLSGGQK 540
ARVNLARAVY QDADIYLLDD PLSAVDAEVS RHLFELCICQ ILHEKITILV THQLQYLKAA 600
SQILILKDGK MVQKGTYTEF LKSGIDFGSL LKKDNEESEQ PPVPGTPTLR NRTFSESSVW 660
SQQSSRPSLK DGALESQDTE NVPVTLSEEN RSEGKVGFQA YKNYFRAGAH WIVFIFLILL 720
NTAAQVAYVL QDWWLSYWAN KQSMLNVTVN GGGNVTEKLD LNWYLGIYSG LTVATVLFGI 780
ARSLLVFYVL VNSSQTLHNK MFESILKAPV LFFDRNPIGR ILNRFSKDIG HLDDLLPLTF 840
LDFIQTLLQV VGVVSVAVAV IPWIAIPLVP LGIIFIFLRR YFLETSRDVK RLESTTRSPV 900
FSHLSSSLQG LWTIRAYKAE ERCQELFDAH QDLHSEAWFL FLTTSRWFAV RLDAICAMFV 960
IIVAFGSLIL AKTLDAGQVG LALSYALTLM GMFQWCVRQS AEVENMMISV ERVIEYTDLE 1020
KEAPWEYQKR PPPAWPHEGV IIFDNVNFMY SPGGPLVLKH LTALIKSQEK VGIVGRTGAG 1080
KSSLISALFR LSEPEGKIWI DKILTTEIGL HDLRKKMSII PQEPVLFTGT MRKNLDPFNE 1140
HTDEELWNAL QEVQLKETIE DLPGKMDTEL AESGSNFSVG QRQLVCLARA ILRKNQILII 1200
DEATANVDPR TDELIQKKIR EKFAHCTVLT IAHRLNTIID SDKIMVLDSG RLKEYDEPYV 1260
LLQNKESLFY KMVQQLGKAE AAALTETAKQ VYFKRNYPHI GHTDHMVTNT SNGQPSTLTI 1320
FETAL 1325 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IDA:MGI.
 GO:0031088; C:platelet dense granule membrane; IDA:MGI.
 GO:0016404; F:15-hydroxyprostaglandin dehydrogenase (NAD+) activity; NAS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0042626; F:ATPase activity, coupled to transmembrane movement of substances; IEA:InterPro.
 GO:0006200; P:ATP catabolic process; IEA:GOC.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0002576; P:platelet degranulation; TAS:Reactome.
 GO:0055085; P:transmembrane transport; TAS:Reactome. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003439; ABC_transporter-like.
 IPR017871; ABC_transporter_CS.
 IPR017940; ABC_transporter_type1.
 IPR001140; ABC_transptr_TM_dom.
 IPR011527; ABC_transptrTM_dom_typ1.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00664; ABC_membrane
 PF00005; ABC_tran 
SMART
 SM00382; AAA 
PROSITE
 PS50929; ABC_TM1F
 PS00211; ABC_TRANSPORTER_1
 PS50893; ABC_TRANSPORTER_2 
PRINTS