CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023465
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Synembryn 
Protein Synonyms/Alias
 Protein Ric-8 
Gene Name
 ric8a 
Gene Synonyms/Alias
 l(1)G0397; Ric8; CG15797 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
539LRQGGIVKPAMIDKDacetylation[1]
Reference
 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702] 
Functional Description
 Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins independently of G- protein coupled receptors. Acts by exchanging bound GDP for free GTP. Plays a key role in asymmetric spindle positioning, a step for asymmetric cell division that generates cell diversity during development by activating G(i) alpha protein independently of G- protein coupled receptors. In addition to its GEF activity, it plays an essential role in cortical subcellular localization of heterotrimeric G proteins, suggesting it acts as a facilitator of G-alpha function through control of its membrane targeting and/or assembling of associated components rather than a GEF. Also required during gastrulation and sensory organ precursors (SOP) formation. 
Sequence Annotation
 MOD_RES 477 477 Phosphoserine.
 MOD_RES 478 478 Phosphoserine.
 MOD_RES 480 480 Phosphoserine.
 MOD_RES 483 483 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Developmental protein; Gastrulation; Guanine-nucleotide releasing factor; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 573 AA 
Protein Sequence
METEHLKRLE AKEADHIPAI LDEFNTKNAD LLVFDSFRTD NLWHELWLAI FGILDDQRLS 60
HLHTQCLNTV RILTRDEFSL QTNYIEQEVN TLLKLARIEA GSLKLPATPD ELKQEEREEP 120
QLEPSQAQSE VIAEALKCLC NLVYQSSDCR RQCLRQHCLD AILKRVASSM RHPCALEYYD 180
MKLLFLLTAL EPAARSRLQI DLNGLTYMTK WLDDKLGEDS VGEEQLNIIC ELLKVMFNVT 240
SAPDKSPNEY EIQSLHLTGV LRELLLRFGD LATEKDRAVV THAINLLTNI SGSCLTELTL 300
RCSNAELESH KEREQDNEKE KDTEAGAGAK PRECCSQCFE KRNVRSLDVL LRYLRQSLAQ 360
QEAEASSHEL LSPVLTVLVK CARSDRVMRH YLRQEILPPL RDVSQRPEVG QELRNHLCRF 420
LTLPAMILRD LSAELLFVLC KENVGRMIKY TGYGNAAGLF AKRGILDCRR VEGTDYSSDS 480
EDSDTEEYKQ QQQGINPVLG CVEPRSKSHL DDISEEQKEY EAMQLVNLIE QLRQGGIVKP 540
AMIDKDGRPQ PLEHILQLQE ELPQQQLDQK RKT 573 
Gene Ontology
 GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
 GO:0008356; P:asymmetric cell division; IMP:UniProtKB.
 GO:0008105; P:asymmetric protein localization; IMP:UniProtKB.
 GO:0040001; P:establishment of mitotic spindle localization; IMP:UniProtKB.
 GO:0007163; P:establishment or maintenance of cell polarity; IMP:UniProtKB.
 GO:0010004; P:gastrulation involving germ band extension; IMP:UniProtKB.
 GO:0055057; P:neuroblast division; IMP:UniProtKB.
 GO:0050821; P:protein stabilization; IMP:UniProtKB. 
Interpro
 IPR016024; ARM-type_fold.
 IPR019318; Gua_nucleotide_exch_fac_Ric8.
 IPR008376; Synembryn. 
Pfam
 PF10165; Ric8 
SMART
  
PROSITE
  
PRINTS
 PR01802; SYNEMBRYN.