UniProt Accession

 RASF1_HUMAN; Q9NS23; B7ZLL1; O14571; O60539; O60710; Q0VGC6; Q5TZT2; Q9HB04; Q9HB18; Q9NS22; Q9UND4; Q9UND5 

Genbank Protein ID

 AF061836; AF132675; AF132676; AF132677; AF040703; AF102770; AF102771; AF102772; BT020047; BT020048; AF286217; AF291719; AC002455; AC002481; CH471055; CH471055; CH471055; BC110412; BC143879 

Genbank Nucleotide ID

 AAC16001.1; AAD44174.1; AAD44175.1; AAD44176.1; AAC70910.2; AAF35127.2; AAF35128.2; AAF35129.2; AAV38850.1; AAV38851.1; AAG10038.1; AAG10064.1; AAB67312.1; EAW65098.1; EAW65101.1; EAW65102.1; AAI10413.1; AAI43880.1 

Protein Name

 Ras association domain-containing protein 1 

Protein Synonyms/Alias

  

Gene Name

 RASSF1 

Gene Synonyms/Alias

 RDA32 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
216	YLPKDAVKHLHVLSR	ubiquitination	[1]
236	VIEALLRKFLVVDDP	ubiquitination	[2]
262	HGQVYLRKLLDDEQP	ubiquitination	[1]
281	LLAGPSDKALSFVLK	ubiquitination	[1, 3]
326	HLRQILQKYSYCRQK	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Potential tumor suppressor. Required for death receptor- dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage. 

Sequence Annotation

 DOMAIN 198 292 Ras-associating.
 DOMAIN 294 341 SARAH.
 ZN_FING 51 105 Phorbol-ester/DAG-type.
 REGION 2 119 Mediates interaction with E4F1.
 REGION 315 318 MOAP1-binding.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Alternative promoter usage; Alternative splicing; Cell cycle; Complete proteome; Cytoplasm; Cytoskeleton; Metal-binding; Microtubule; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Tumor suppressor; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 344 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0015630; C:microtubule cytoskeleton; IDA:UniProtKB.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0008270; F:zinc ion binding; TAS:UniProtKB.
 GO:0007050; P:cell cycle arrest; IMP:UniProtKB.
 GO:0071157; P:negative regulation of cell cycle arrest; IDA:UniProtKB.
 GO:0031398; P:positive regulation of protein ubiquitination; IDA:UniProtKB.
 GO:0050821; P:protein stabilization; IDA:UniProtKB.
 GO:0007265; P:Ras protein signal transduction; IDA:UniProtKB.
 GO:0006974; P:response to DNA damage stimulus; IMP:UniProtKB. 

Interpro

 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000159; Ras-assoc.
 IPR011524; SARAH_dom. 

Pfam

 PF00130; C1_1
 PF00788; RA 

SMART

 SM00109; C1
 SM00314; RA 

PROSITE

 PS50200; RA
 PS50951; SARAH
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 

PRINTS