CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044809
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Mothers against decapentaplegic homolog 4 
Protein Synonyms/Alias
  
Gene Name
 SMAD4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
289RARLHIGKGVQLECKubiquitination[1, 2]
332APGDAVHKIYPSAYIacetylation[2, 3]
423DYPRQSIKETPCWIEubiquitination[1, 2, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Nucleus; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 456 AA 
Protein Sequence
MDNMSITNTP TSNDACLSIV HSLMCHRQGG ESETFAKRAI ESLVKKLKEK KDELDSLITA 60
ITTNGAHPSK CVTIQRTLDG RLQVAGRKGF PHVIYARLWR WPDLHKNELK HVKYCQYAFD 120
LKCDSVCVNP YHYERVVSPG IDLSGLTLQS NAPSSMMVKD EYVHDFEGQP SLSTEGHSIQ 180
TIQHPPSNRA STETYSTPAL LAPSESNATS TANFPNIPVA STTPEYWCSI AYFEMDVQVG 240
ETFKVPSSCP IVTVDGYVDP SGGDRFCLGQ LSNVHRTEAI ERARLHIGKG VQLECKGEGD 300
VWVRCLSDHA VFVQSYYLDR EAGRAPGDAV HKIYPSAYIK VFDLRQCHRQ MQQQAATAQA 360
AAAAQAAAVA GNIPGPGSVG GIAPAISLSA AAGIGVDDLR RLCILRMSFV KGWGPDYPRQ 420
SIKETPCWIE IHLHRALQLL DEVLHTMPIA DPQPLD 456 
Gene Ontology
 GO:0005667; C:transcription factor complex; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; IEA:InterPro. 
Interpro
 IPR013790; Dwarfin.
 IPR003619; MAD_homology1_Dwarfin-type.
 IPR013019; MAD_homology_MH1.
 IPR017855; SMAD_dom-like.
 IPR001132; SMAD_dom_Dwarfin-type.
 IPR008984; SMAD_FHA_domain. 
Pfam
 PF03165; MH1
 PF03166; MH2 
SMART
 SM00523; DWA
 SM00524; DWB 
PROSITE
 PS51075; MH1
 PS51076; MH2 
PRINTS