UniProt Accession

 LMAN1_HUMAN; P49257; Q12895; Q8N5I7; Q9UQG1; Q9UQG2; Q9UQG3; Q9UQG4; Q9UQG5; Q9UQG6; Q9UQG7; Q9UQG8; Q9UQG9; Q9UQH0; Q9UQH1; Q9UQH2 

Genbank Protein ID

 X71661; U09716; AF081866; AF081865; AF081867; AF081869; AF081868; AF081871; AF081870; AF081873; AF081872; AF081875; AF081874; AF081877; AF081876; AF081879; AF081878; AF081880; AF081882; AF081881; AF081884; AF081883; AF081885; BC032330 

Genbank Nucleotide ID

 CAA50653.1; AAA95960.1; AAD32479.1; AAD32479.1; AAD32480.1; AAD32481.1; AAD32481.1; AAD32482.1; AAD32482.1; AAD32483.1; AAD32483.1; AAD32484.1; AAD32484.1; AAD32485.1; AAD32485.1; AAD32486.1; AAD32486.1; AAD32487.1; AAD32488.1; AAD32488.1; AAD32489.1; AAD32489.1; AAD32490.1; AAH32330.1 

Protein Name

 Protein ERGIC-53 

Protein Synonyms/Alias

 ER-Golgi intermediate compartment 53 kDa protein; Gp58; Intracellular mannose-specific lectin MR60; Lectin mannose-binding 1 

Gene Name

 LMAN1 

Gene Synonyms/Alias

 ERGIC53; F5F8D 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
49	PHRRFEYKYSFKGPH	ubiquitination	[1]
87	IRVAPSLKSQRGSVW	ubiquitination	[1]
346	NRIHLEIKQLNRQLD	ubiquitination	[1, 2, 3, 4, 5, 6]
372	SLTEEISKRGAGMPG	ubiquitination	[1, 2, 5, 6]
407	LRQVNEMKNSMSETV	ubiquitination	[1, 6]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins. 

Sequence Annotation

 DOMAIN 44 267 L-type lectin-like.
 REGION 251 253 Carbohydrate binding (By similarity).
 MOTIF 509 510 ER export motif.
 METAL 152 152 Calcium.
 METAL 154 154 Calcium; via carbonyl oxygen.
 METAL 156 156 Calcium.
 METAL 181 181 Calcium.
 BINDING 88 88 Carbohydrate (By similarity).
 BINDING 121 121 Carbohydrate (By similarity).
 BINDING 156 156 Carbohydrate (By similarity).
 BINDING 178 178 Carbohydrate (By similarity).
 DISULFID 190 230
 DISULFID 466 466 Interchain.
 DISULFID 475 475 Interchain.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus; Lectin; Membrane; Metal-binding; Polymorphism; Protein transport; Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 510 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; TAS:ProtInc.
 GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IDA:UniProtKB.
 GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
 GO:0012507; C:ER to Golgi transport vesicle membrane; TAS:Reactome.
 GO:0000139; C:Golgi membrane; TAS:ProtInc.
 GO:0016021; C:integral to membrane; TAS:ProtInc.
 GO:0030017; C:sarcomere; IEA:Compara.
 GO:0005537; F:mannose binding; TAS:ProtInc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0051082; F:unfolded protein binding; TAS:ProtInc.
 GO:0007596; P:blood coagulation; TAS:ProtInc.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; TAS:ProtInc.
 GO:0007030; P:Golgi organization; IMP:UniProtKB.
 GO:0010638; P:positive regulation of organelle organization; IMP:UniProtKB.
 GO:0043687; P:post-translational protein modification; TAS:Reactome.
 GO:0006457; P:protein folding; TAS:ProtInc.
 GO:0018279; P:protein N-linked glycosylation via asparagine; TAS:Reactome.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 

Interpro

 IPR008985; ConA-like_lec_gl_sf.
 IPR013320; ConA-like_subgrp.
 IPR005052; Lectin_leg. 

Pfam

 PF03388; Lectin_leg-like 

SMART

  

PROSITE

 PS51328; L_LECTIN_LIKE 

PRINTS