CPLM-011364

Genbank Nucleotide ID

m7GpppX diphosphatase

Protein Synonyms/Alias

DCS-1; Hint-related 7meGMP-directed hydrolase 1; Protein Dcs1p; Scavenger mRNA-decapping enzyme DcpS

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Position	Peptide	Type	References
112	MESNPTAKLNLIWPA	ubiquitination	[1]
126	ATPIHIKKYEQQNFH	acetylation	[2]
126	ATPIHIKKYEQQNFH	ubiquitination	[1]

[1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
[2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]

Functional Description

Decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by the 3'->5' exosome-mediated mRNA decay pathway. Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG) and tri-methyl guanosine nucleoside triphosphate (m3(2,2,7)GpppG) with up to 10 nucleotide substrates (small capped oligoribonucleotides) and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP) or tri- methyl guanosine nucleoside monophosphate (m3(2,2,7)GMP), respectively. Does not hydrolyze unmethylated cap analog (GpppG) and shows no decapping activity on intact m7GpppG-capped mRNA molecules longer than 25 nucleotides. Does not hydrolyze 7- methylguanosine diphosphate (m7GDP) and tri-methylguanosine diphosphate (m3(2,2,7)GDP) to (m(7)GMP) and m3(2,2,7)GMP, respectively (PubMed:22985415). May also play a role in the 5'->3 mRNA decay pathway; m7GDP, the downstream product released by the 5'->3' mRNA mediated decapping activity, may be also converted by DCS1 to m7GMP (PubMed:14523240). Binds to m7GpppG and strongly to m7GDP. May also regulates the 5'->3' exoribonucleolytic mRNA decay pathway in a cap-independent manner. Negatively regulates trehalase activity.

REGION 259 270 Substrate binding (By similarity).
MOTIF 266 270 Histidine triad motif (By similarity).
ACT_SITE 268 268 Nucleophile (By similarity).
BINDING 171 171 Substrate (By similarity).
BINDING 196 196 Substrate (By similarity).
MOD_RES 60 60 Phosphoserine.
MOD_RES 66 66 Phosphothreonine; alternate.
MOD_RES 66 66 Phosphothreonine; by YAK1; alternate
MOD_RES 70 70 Phosphotyrosine.
MOD_RES 120 120 Phosphothreonine.

Complete proteome; Cytoplasm; Hydrolase; Phosphoprotein; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0000932; C:cytoplasmic mRNA processing body; IDA:SGD.
GO:0005739; C:mitochondrion; IDA:SGD.
GO:0005634; C:nucleus; IDA:SGD.
GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
GO:0044692; F:exoribonuclease activator activity; IDA:SGD.
GO:0050072; F:m7G(5')pppN diphosphatase activity; IDA:UniProtKB.
GO:0046982; F:protein heterodimerization activity; IDA:UniProtKB.
GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
GO:0000340; F:RNA 7-methylguanosine cap binding; IDA:UniProtKB.
GO:0000290; P:deadenylation-dependent decapping of nuclear-transcribed mRNA; IDA:SGD.
GO:0031086; P:nuclear-transcribed mRNA catabolic process, deadenylation-independent decay; IMP:SGD.
GO:1901919; P:positive regulation of exoribonuclease activity; IDA:SGD.
GO:0090342; P:regulation of cell aging; IMP:UniProtKB.
GO:0009408; P:response to heat; IDA:UniProtKB.
GO:0007584; P:response to nutrient; IDA:UniProtKB.
GO:0006970; P:response to osmotic stress; IDA:UniProtKB.
GO:0006979; P:response to oxidative stress; IDA:UniProtKB.

IPR008594; DcpS/DCS2.
IPR019808; Histidine_triad_CS.
IPR011146; HIT-like.
IPR011145; Scavenger_mRNA_decap_enz_N.