UniProt Accession

 NFM_RAT; P12839; Q63370 

Genbank Protein ID

 M18628; Z12152 

Genbank Nucleotide ID

 AAA41696.1; CAA78136.1 

Protein Name

 Neurofilament medium polypeptide 

Protein Synonyms/Alias

 NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein 

Gene Name

 Nefm 

Gene Synonyms/Alias

 Nef3; Nfm 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
373	ENELRGTKWEMARHL	acetylation	[1]
402	IEIAAYRKLLEGEET	ubiquitination	[2]
682	EEMITITKSVKVSLE	ubiquitination	[2]
685	ITITKSVKVSLEKDT	ubiquitination	[2]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. 

Sequence Annotation

 REGION 2 104 Head.
 REGION 104 411 Rod.
 REGION 104 135 Coil 1A.
 REGION 136 148 Linker 1.
 REGION 149 247 Coil 1B.
 REGION 248 264 Linker 12.
 REGION 265 286 Coil 2A.
 REGION 287 290 Linker 2.
 REGION 291 411 Coil 2B.
 REGION 412 845 Tail.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 319 319 Phosphotyrosine (By similarity).
 MOD_RES 503 503 Phosphoserine.
 MOD_RES 507 507 Phosphoserine.
 MOD_RES 537 537 Phosphoserine.
 MOD_RES 545 545 Phosphoserine (By similarity).
 MOD_RES 551 551 Phosphoserine (By similarity).
 MOD_RES 604 604 Phosphoserine.
 MOD_RES 609 609 Phosphoserine.
 MOD_RES 643 643 Phosphoserine (By similarity).
 MOD_RES 667 667 Phosphoserine.
 MOD_RES 687 687 Phosphoserine (By similarity).
 MOD_RES 713 713 Phosphoserine (By similarity).
 MOD_RES 767 767 Phosphoserine (By similarity).
 CARBOHYD 48 48 O-linked (GlcNAc).
 CARBOHYD 431 431 O-linked (GlcNAc).  

Keyword

 Acetylation; Coiled coil; Complete proteome; Direct protein sequencing; Glycoprotein; Intermediate filament; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 846 AA 

Protein Sequence

Gene Ontology

 GO:0030424; C:axon; IDA:BHF-UCL.
 GO:0005856; C:cytoskeleton; IDA:UniProtKB.
 GO:0005882; C:intermediate filament; IEA:UniProtKB-KW.
 GO:0005198; F:structural molecule activity; IEA:InterPro. 

Interpro

 IPR016044; F.
 IPR001664; IF.
 IPR006821; Intermed_filament_DNA-bd.
 IPR018039; Intermediate_filament_CS.
 IPR002957; Keratin_I. 

Pfam

 PF00038; Filament
 PF04732; Filament_head 

SMART

  

PROSITE

 PS00226; IF 

PRINTS

 PR01248; TYPE1KERATIN.