CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008558
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 F-actin-capping protein subunit alpha-1 
Protein Synonyms/Alias
 CapZ alpha-1 
Gene Name
 CAPZA1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
13DRVSDEEKVRIAAKFubiquitination[1]
19EKVRIAAKFITHAPPacetylation[2, 3, 4, 5]
19EKVRIAAKFITHAPPubiquitination[1, 6, 7, 8, 9, 10]
66MDQFTPVKIEGYEDQubiquitination[8]
97PRNKISFKFDHLRKEacetylation[2, 3]
97PRNKISFKFDHLRKEubiquitination[6]
103FKFDHLRKEASDPQPubiquitination[7, 8]
118EEADGGLKSWRESCDubiquitination[1, 5, 6, 7, 8, 9, 10]
133SALRAYVKDHYSNGFubiquitination[1, 5, 6, 8]
166ESHQFQPKNFWNGRWubiquitination[1, 6, 8]
178GRWRSEWKFTITPPTubiquitination[1, 5, 6, 9]
210NVQLVSHKDVQDSLTubiquitination[1]
226SNEAQTAKEFIKIIEubiquitination[6, 7, 8, 10]
256TMSDTTFKALRRQLPubiquitination[7, 8]
268QLPVTRTKIDWNKILubiquitination[10, 11]
273RTKIDWNKILSYKIGubiquitination[6, 7, 9, 10, 11]
278WNKILSYKIGKEMQNubiquitination[6, 7]
281ILSYKIGKEMQNA**ubiquitination[7, 11]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [10] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [11] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. 
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 19 19 N6-acetyllysine.
 MOD_RES 97 97 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Actin capping; Actin-binding; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 286 AA 
Protein Sequence
MADFDDRVSD EEKVRIAAKF ITHAPPGEFN EVFNDVRLLL NNDNLLREGA AHAFAQYNMD 60
QFTPVKIEGY EDQVLITEHG DLGNSRFLDP RNKISFKFDH LRKEASDPQP EEADGGLKSW 120
RESCDSALRA YVKDHYSNGF CTVYAKTIDG QQTIIACIES HQFQPKNFWN GRWRSEWKFT 180
ITPPTAQVVG VLKIQVHYYE DGNVQLVSHK DVQDSLTVSN EAQTAKEFIK IIENAENEYQ 240
TAISENYQTM SDTTFKALRR QLPVTRTKID WNKILSYKIG KEMQNA 286 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005576; C:extracellular region; TAS:Reactome.
 GO:0008290; C:F-actin capping protein complex; TAS:ProtInc.
 GO:0071203; C:WASH complex; IDA:UniProtKB.
 GO:0003779; F:actin binding; TAS:ProtInc.
 GO:0030036; P:actin cytoskeleton organization; IEA:InterPro.
 GO:0051693; P:actin filament capping; IEA:UniProtKB-KW.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0006928; P:cellular component movement; TAS:UniProtKB.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0006461; P:protein complex assembly; TAS:ProtInc. 
Interpro
 IPR017865; F-actin_cap_asu_CS.
 IPR002189; WASH_F-actin_cap_alpha. 
Pfam
 PF01267; F-actin_cap_A 
SMART
  
PROSITE
 PS00748; F_ACTIN_CAPPING_A_1
 PS00749; F_ACTIN_CAPPING_A_2 
PRINTS
 PR00191; FACTINCAPA.