CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044849
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Calreticulin 
Protein Synonyms/Alias
  
Gene Name
 CALR 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
24AEPAVYFKEQFLDGDubiquitination[1, 2]
48KHKSDFGKFVLSSGKacetylation[3]
48KHKSDFGKFVLSSGKubiquitination[1, 4]
55KFVLSSGKFYGDEEKubiquitination[1, 2]
75ICGPGTKKVHVIFNYubiquitination[2, 4]
83VHVIFNYKGKNVLINacetylation[5]
83VHVIFNYKGKNVLINubiquitination[4]
85VIFNYKGKNVLINKDubiquitination[1, 2, 4]
91GKNVLINKDIRCKDDacetylation[6]
91GKNVLINKDIRCKDDubiquitination[1, 2, 4, 7]
138DWDFLPPKKIKDPDAubiquitination[2]
139WDFLPPKKIKDPDASubiquitination[4]
141FLPPKKIKDPDASKPacetylation[3, 6, 7]
141FLPPKKIKDPDASKPubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [6] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Chaperone; Complete proteome; Endoplasmic reticulum; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 247 AA 
Protein Sequence
MLLSVPLLLG LLGLAVAEPA VYFKEQFLDG DGWTSRWIES KHKSDFGKFV LSSGKFYGDE 60
EKDKGPDICG PGTKKVHVIF NYKGKNVLIN KDIRCKDDEF THLYTLIVRP DNTYEVKIDN 120
SQVESGSLED DWDFLPPKKI KDPDASKPED WDERAKIDDP TDSKPEDWDK PEHIPDPDAK 180
KPEDWDEEMD GEWEPPVIQN PEYKGEWKPR QIDNPDYKGT WIHPEIDNPE YSPDPSIYAY 240
DNFGVLG 247 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0006457; P:protein folding; IEA:InterPro. 
Interpro
 IPR001580; Calret/calnex.
 IPR018124; Calret/calnex_CS.
 IPR009033; Calreticulin/calnexin_P_dom.
 IPR008985; ConA-like_lec_gl_sf.
 IPR013320; ConA-like_subgrp. 
Pfam
 PF00262; Calreticulin 
SMART
  
PROSITE
 PS00805; CALRETICULIN_REPEAT 
PRINTS
 PR00626; CALRETICULIN.