| Tag | Content |
|---|
| CPLM ID | CPLM-002892 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Acetate kinase |
Protein Synonyms/Alias | Acetokinase |
Gene Name | ackA |
Gene Synonyms/Alias | ack; b2296; JW2293 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 45 | HLPEARIKWKMDGNK | acetylation | [1] | | 47 | PEARIKWKMDGNKQE | acetylation | [1] | | 52 | KWKMDGNKQEAALGA | acetylation | [1] | | 98 | RIVHGGEKYTSSVVI | acetylation | [1] | | 136 | IGIEEALKSFPQLKD | acetylation | [1] | | 142 | LKSFPQLKDKNVAVF | acetylation | [1] | | 224 | VSAIRNGKCVDTSMG | acetylation | [1] | | 272 | AINKLLTKESGLLGL | acetylation | [1, 2] | | 296 | VEDNYATKEDAKRAM | acetylation | [1, 2] | | 312 | VYCHRLAKYIGAYTA | acetylation | [1] | | 348 | VRELSLGKLGVLGFE | acetylation | [1] | | 368 | NLAARFGKSGFINKE | acetylation | [1] | | 374 | GKSGFINKEGTRPAV | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli. Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z. J Proteome Res. 2013 Feb 1;12(2):844-51. [ PMID: 23294111] |
Functional Description | Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically. |
Sequence Annotation | NP_BIND 210 214 ATP (By similarity).
NP_BIND 285 287 ATP (By similarity).
NP_BIND 333 337 ATP (By similarity).
ACT_SITE 150 150 Proton donor/acceptor (By similarity).
METAL 10 10 Magnesium (By similarity).
METAL 387 387 Magnesium (By similarity).
BINDING 17 17 ATP (By similarity).
BINDING 91 91 Substrate (By similarity). |
Keyword | 3D-structure; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 400 AA |
Protein Sequence | MSSKLVLVLN CGSSSLKFAI IDAVNGEEYL SGLAECFHLP EARIKWKMDG NKQEAALGAG 60
AAHSEALNFI VNTILAQKPE LSAQLTAIGH RIVHGGEKYT SSVVIDESVI QGIKDAASFA 120
PLHNPAHLIG IEEALKSFPQ LKDKNVAVFD TAFHQTMPEE SYLYALPYNL YKEHGIRRYG 180
AHGTSHFYVT QEAAKMLNKP VEELNIITCH LGNGGSVSAI RNGKCVDTSM GLTPLEGLVM 240
GTRSGDIDPA IIFHLHDTLG MSVDAINKLL TKESGLLGLT EVTSDCRYVE DNYATKEDAK 300
RAMDVYCHRL AKYIGAYTAL MDGRLDAVVF TGGIGENAAM VRELSLGKLG VLGFEVDHER 360
NLAARFGKSG FINKEGTRPA VVIPTNEELV IAQDASRLTA 400 |
Gene Ontology | GO:0005829; C:cytosol; IDA:UniProtKB. GO:0016020; C:membrane; IDA:UniProtKB. GO:0008776; F:acetate kinase activity; IDA:EcoliWiki. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0000287; F:magnesium ion binding; IEA:HAMAP. GO:0008270; F:zinc ion binding; IDA:EcoliWiki. GO:0019413; P:acetate biosynthetic process; IGI:EcoliWiki. GO:0006085; P:acetyl-CoA biosynthetic process; IEA:UniProtKB-UniPathway. GO:0019542; P:propionate biosynthetic process; IGI:EcoliWiki. |
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