UniProt Accession

 MP2K2_MOUSE; Q63932; Q9D7B0 

Genbank Protein ID

 S68267; AK009392; AK080574; CH466553 

Genbank Nucleotide ID

 AAC60678.1; BAB26261.1; BAC37945.1; EDL31456.1 

Protein Name

 Dual specificity mitogen-activated protein kinase kinase 2 

Protein Synonyms/Alias

 MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2 

Gene Name

 Map2k2 

Gene Synonyms/Alias

 Mek2; Mkk2; Prkmk2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
88	GNGGVVTKARHRPSG	ubiquitination	[1]
196	QIMHRDVKPSNILVN	ubiquitination	[1]
362	PAERADLKLLMNHAF	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases. 

Sequence Annotation

 DOMAIN 72 370 Protein kinase.
 NP_BIND 78 86 ATP (By similarity).
 ACT_SITE 194 194 Proton acceptor (By similarity).
 BINDING 101 101 ATP.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 222 222 Phosphoserine; by RAF (By similarity).
 MOD_RES 226 226 Phosphoserine; by RAF (By similarity).
 MOD_RES 293 293 Phosphoserine (By similarity).
 MOD_RES 295 295 Phosphoserine (By similarity).
 MOD_RES 395 395 Phosphothreonine (By similarity).
 MOD_RES 397 397 Phosphothreonine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; Direct protein sequencing; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 401 AA 

Protein Sequence

Gene Ontology

 GO:0005938; C:cell cortex; IEA:Compara.
 GO:0005911; C:cell-cell junction; IEA:Compara.
 GO:0005829; C:cytosol; TAS:UniProtKB.
 GO:0005769; C:early endosome; TAS:UniProtKB.
 GO:0005783; C:endoplasmic reticulum; IEA:Compara.
 GO:0005925; C:focal adhesion; TAS:UniProtKB.
 GO:0005794; C:Golgi apparatus; TAS:UniProtKB.
 GO:0009898; C:internal side of plasma membrane; IEA:Compara.
 GO:0005770; C:late endosome; TAS:UniProtKB.
 GO:0005874; C:microtubule; IEA:Compara.
 GO:0005739; C:mitochondrion; TAS:UniProtKB.
 GO:0005634; C:nucleus; TAS:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0005778; C:peroxisomal membrane; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004708; F:MAP kinase kinase activity; IEA:Compara.
 GO:0032947; F:protein complex scaffold; IDA:BHF-UCL.
 GO:0043539; F:protein serine/threonine kinase activator activity; IEA:Compara.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0004712; F:protein serine/threonine/tyrosine kinase activity; TAS:UniProtKB.
 GO:0004713; F:protein tyrosine kinase activity; IEA:UniProtKB-KW.
 GO:0000187; P:activation of MAPK activity; TAS:UniProtKB.
 GO:0036289; P:peptidyl-serine autophosphorylation; IEA:Compara.
 GO:2000147; P:positive regulation of cell motility; IDA:BHF-UCL.
 GO:2000641; P:regulation of early endosome to late endosome transport; TAS:UniProtKB.
 GO:0090170; P:regulation of Golgi inheritance; TAS:UniProtKB.
 GO:0032872; P:regulation of stress-activated MAPK cascade; TAS:UniProtKB. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS