| Tag | Content |
|---|
| CPLM ID | CPLM-019091 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Alkylated DNA repair protein alkB homolog 8 |
Protein Synonyms/Alias | Probable alpha-ketoglutarate-dependent dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8; tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8 |
Gene Name | ALKBH8 |
Gene Synonyms/Alias | ABH8 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 422 | DIGCGNGKYLGINKE | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis. |
Sequence Annotation | DOMAIN 43 120 RRM.
DOMAIN 220 337 Fe2OG dioxygenase.
REGION 227 229 Alpha-ketoglutarate binding (By
REGION 328 334 Alpha-ketoglutarate binding (By
REGION 411 664 Methyltransferase domain.
METAL 238 238 Iron; catalytic (By similarity).
METAL 240 240 Iron; catalytic (By similarity).
METAL 292 292 Iron; catalytic (By similarity). |
Keyword | 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Iron; Metal-binding; Methyltransferase; Multifunctional enzyme; Nucleus; Oxidoreductase; Reference proteome; RNA-binding; S-adenosyl-L-methionine; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 664 AA |
Protein Sequence | MFAMDSNHQS NYKLSKTEKK FLRKQIKAKH TLLRHEGIET VSYATQSLVV ANGGLGNGVS 60
RNQLLPVLEK CGLVDALLMP PNKPYSFARY RTTEESKRAY VTLNGKEVVD DLGQKITLYL 120
NFVEKVQWKE LRPQALPPGL MVVEEIISSE EEKMLLESVD WTEDTDNQNS QKSLKHRRVK 180
HFGYEFHYEN NNVDKDKPLS GGLPDICESF LEKWLRKGYI KHKPDQMTIN QYEPGQGIPA 240
HIDTHSAFED EIVSLSLGSE IVMDFKHPDG IAVPVMLPRR SLLVMTGESR YLWTHGITCR 300
KFDTVQASES LKSGIITSDV GDLTLSKRGL RTSFTFRKVR QTPCNCSYPL VCDSQRKETP 360
PSFPESDKEA SRLEQEYVHQ VYEEIAGHFS STRHTPWPHI VEFLKALPSG SIVADIGCGN 420
GKYLGINKEL YMIGCDRSQN LVDICRERQF QAFVCDALAV PVRSGSCDAC ISIAVIHHFA 480
TAERRVAALQ EIVRLLRPGG KALIYVWAME QEYNKQKSKY LRGNRNSQGK KEEMNSDTSV 540
QRSLVEQMRD MGSRDSASSV PRINDSQEGG CNSRQVSNSK LPVHVNRTSF YSQDVLVPWH 600
LKGNPDKGKP VEPFGPIGSQ DPSPVFHRYY HVFREGELEG ACRTVSDVRI LQSYYDQGNW 660
CVILQKA 667 |
Gene Ontology | GO:0005829; C:cytosol; IDA:UniProtKB. GO:0015630; C:microtubule cytoskeleton; IDA:HPA. GO:0005634; C:nucleus; IDA:UniProtKB. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0016706; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; IEA:InterPro. GO:0003723; F:RNA binding; IEA:UniProtKB-KW. GO:0016300; F:tRNA (uracil) methyltransferase activity; IDA:UniProtKB. GO:0006974; P:response to DNA damage stimulus; IDA:UniProtKB. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |