UniProt Accession

 SIR2_HUMAN; Q8IXJ6; A8K3V1; B2RB45; O95889; Q924Y7; Q9P0G8; Q9UNT0; Q9Y6E9 

Genbank Protein ID

 AF083107; AF095714; AY030277; AJ505014; AF160214; AK290716; AK314492; CH471126; CH471126; BC003012; BC003547; AF131800 

Genbank Nucleotide ID

 AAD40850.2; AAD45971.1; AAK51133.1; CAD43717.1; AAF67015.1; BAF83405.1; BAG37092.1; EAW56833.1; EAW56835.1; AAH03012.1; AAH03547.1; AAD20046.1 

Protein Name

 NAD-dependent protein deacetylase sirtuin-2 

Protein Synonyms/Alias

 Regulatory protein SIR2 homolog 2; SIR2-like protein 2 

Gene Name

 SIRT2 

Gene Synonyms/Alias

 SIR2L; SIR2L2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
55	TLSLGSQKERLLDEL	ubiquitination	[1, 2]
158	FMRLLKDKGLLLRCY	ubiquitination	[1]
287	TPRLLINKEKAGQSD	ubiquitination	[3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and non-histone proteins. Deacetylates 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Deacetylates PCK1, opposing proteasomal degradation. Deacetylates 'Lys-310' of RELA. 

Sequence Annotation

 DOMAIN 65 340 Deacetylase sirtuin-type.
 NP_BIND 84 104 NAD (By similarity).
 NP_BIND 167 170 NAD (By similarity).
 NP_BIND 261 263 NAD (By similarity).
 NP_BIND 286 288 NAD (By similarity).
 ACT_SITE 187 187 Proton acceptor.
 METAL 195 195 Zinc.
 METAL 200 200 Zinc.
 METAL 221 221 Zinc.
 METAL 224 224 Zinc.
 BINDING 324 324 NAD; via amide nitrogen (By similarity).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 23 23 Phosphoserine (By similarity).  

Keyword

 3D-structure; Acetylation; Alternative splicing; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Hydrolase; Metal-binding; Microtubule; Mitosis; NAD; Phosphoprotein; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 389 AA 

Protein Sequence

Gene Ontology

 GO:0005677; C:chromatin silencing complex; NAS:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005874; C:microtubule; IDA:UniProtKB.
 GO:0070403; F:NAD+ binding; IDA:UniProtKB.
 GO:0017136; F:NAD-dependent histone deacetylase activity; IDA:UniProtKB.
 GO:0042903; F:tubulin deacetylase activity; IDA:UniProtKB.
 GO:0043130; F:ubiquitin binding; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IDA:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0000183; P:chromatin silencing at rDNA; NAS:UniProtKB.
 GO:0006348; P:chromatin silencing at telomere; NAS:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0045843; P:negative regulation of striated muscle tissue development; IDA:UniProtKB.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IMP:UniProtKB.
 GO:0006471; P:protein ADP-ribosylation; NAS:UniProtKB.
 GO:0007096; P:regulation of exit from mitosis; NAS:UniProtKB.
 GO:0042325; P:regulation of phosphorylation; NAS:UniProtKB.
 GO:0051775; P:response to redox state; NAS:UniProtKB. 

Interpro

 IPR017328; NAD-dep_deAcase_SIR2_class_I.
 IPR003000; Sirtuin.
 IPR026590; Ssirtuin_cat_dom. 

Pfam

 PF02146; SIR2 

SMART

  

PROSITE

 PS50305; SIRTUIN 

PRINTS