CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020505
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 
Protein Synonyms/Alias
 Complex I-B8; CI-B8; NADH-ubiquinone oxidoreductase B8 subunit 
Gene Name
 Ndufa2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
13ASRAVGAKLGLREIRacetylation[1, 2, 3, 4]
64ECSEVQPKLWARYAFacetylation[1, 2, 3, 4, 5, 6]
64ECSEVQPKLWARYAFsuccinylation[6]
75RYAFGQEKTVSLNNLacetylation[1, 2, 3, 4, 5, 7]
98MQNVLSGKA******acetylation[3]
Reference
 [1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [6] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
 Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). 
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 64 64 N6-acetyllysine.
 MOD_RES 75 75 N6-acetyllysine.
 DISULFID 24 58 Redox-active (By similarity).  
Keyword
 Acetylation; Complete proteome; Direct protein sequencing; Disulfide bond; Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Respiratory chain; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 99 AA 
Protein Sequence
MAAAAASRAV GAKLGLREIR VHLCQRSPGS QGVRDFIVQR YVELKKAHPN LPILIRECSE 60
VQPKLWARYA FGQEKTVSLN NLSADEVTRA MQNVLSGKA 99 
Gene Ontology
 GO:0005747; C:mitochondrial respiratory chain complex I; TAS:MGI.
 GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; TAS:MGI.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW. 
Interpro
 IPR016464; NADH_Ub_cplx-1_asu_su-2.
 IPR007741; Ribosome/NADH_DH.
 IPR012336; Thioredoxin-like_fold. 
Pfam
 PF05047; L51_S25_CI-B8 
SMART
 SM00916; L51_S25_CI-B8 
PROSITE
  
PRINTS