CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022546
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Inhibitor of Bruton tyrosine kinase 
Protein Synonyms/Alias
 IBtk 
Gene Name
 IBTK 
Gene Synonyms/Alias
 BTKI; KIAA1417 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
11PMPDCTSKCRSLKHAubiquitination[1]
655KPRIHLNKNPEEYQGubiquitination[1]
687KSAFEVYKSNQAQTVubiquitination[1, 2]
1213DFLLEEKKSVTSHSSubiquitination[1]
1350LAVPMWNKHGC****ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription. 
Sequence Annotation
 REPEAT 51 80 ANK 1.
 REPEAT 85 114 ANK 2.
 REPEAT 141 194 RCC1 1.
 REPEAT 195 246 RCC1 2.
 REPEAT 248 301 RCC1 3.
 DOMAIN 564 644 BTB 1.
 DOMAIN 768 836 BTB 2.
 REPEAT 806 835 ANK 3.
 MOD_RES 990 990 Phosphoserine.
 MOD_RES 1004 1004 Phosphoserine.
 MOD_RES 1045 1045 Phosphoserine.
 MOD_RES 1054 1054 Phosphoserine.
 MOD_RES 1113 1113 Phosphoserine.
 MOD_RES 1116 1116 Phosphoserine.  
Keyword
 Alternative splicing; ANK repeat; Complete proteome; Cytoplasm; Membrane; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1353 AA 
Protein Sequence
MSSPMPDCTS KCRSLKHALD VLSVVTKGSE NQIKAFLSSH CYNAATIKDV FGRNALHLVS 60
SCGKKGVLDW LIQKGVDLLV KDKESGWTAL HRSIFYGHID CVWSLLKHGV SLYIQDKEGL 120
SALDLVMKDR PTHVVFKNTD PTDVYTWGDN TNFTLGHGSQ NSKHHPELVD LFSRSGIYIK 180
QVVLCKFHSV FLSQKGQVYT CGHGPGGRLG HGDEQTCLVP RLVEGLNGHN CSQVAAAKDH 240
TVVLTEDGCV YTFGLNIFHQ LGIIPPPSSC NVPRQIQAKY LKGRTIIGVA AGRFHTVLWT 300
REAVYTMGLN GGQLGCLLDP NGEKCVTAPR QVSALHHKDI ALSLVAASDG ATVCVTTRGD 360
IYLLADYQCK KMASKQLNLK KVLVSGGHME YKVDPEHLKE NGGQKICILA MDGAGRVFCW 420
RSVNSSLKQC RWAYPRQVFI SDIALNRNEI LFVTQDGEGF RGRWFEEKRK SSEKKEILSN 480
LHNSSSDVSY VSDINSVYER IRLEKLTFAH RAVSVSTDPS GCNFAILQSD PKTSLYEIPA 540
VSSSSFFEEF GKLLREADEM DSIHDVTFQV GNRLFPAHKY ILAVHSDFFQ KLFLSDGNTS 600
EFTDIYQKDE DSAGCHLFVV EKVHPDMFEY LLQFIYTDTC DFLTHGFKPR IHLNKNPEEY 660
QGTLNSHLNK VNFHEDDNQK SAFEVYKSNQ AQTVSERQKS KPKSCKKGKN IREDDPVRML 720
QTVAKKFDFS NLSSRLDGVR FENEKINVIA KNTGNKLKLS QKKCSFLCDV TMKSVDGKEF 780
PCHKCVLCAR LEYFHSMLSS SWIEASSCAA LEMPIHSDIL KVILDYLYTD EAVVIKESQN 840
VDFICSVLVV ADQLLITRLK EICEVALTEK LTLKNAAMLL EFAAMYSAKQ LKLSCLQFIG 900
LNMAALLEAR SLDVLSDGVL KDLSEFYRKM IPAMDRRVIT PYQDGPDISY LEVEDGDIFL 960
KEEINMEQNH SETMFKKAKT KAKKKPRKRS DSSGGYNLSD IIQSPSSTGL LKSGKTNSVE 1020
SLPELLTSDS EGSYAGVGSP RDLQSPDFTT GFHSDKIEAK VKPYVNGTSP VYSREDLKPW 1080
EKSPILKISA PQPIPSNRID TTSSASWVAG SFSPVSPPVV DLRTIMEIEE SRQKCGATPK 1140
SHLGKTVSHG VKLSQKQRKM IALTTKENNS GMNSMETVLF TPSKAPKPVN AWASSLHSVS 1200
SKSFRDFLLE EKKSVTSHSS GDHVKKVSFK GIENSQAPKI VRCSTHGTPG PEGNHISDLP 1260
LLDSPNPWLS SSVTAPSMVA PVTFASIVEE ELQQEAALIR SREKPLALIQ IEEHAIQDLL 1320
VFYEAFGNPE EFVIVERTPQ GPLAVPMWNK HGC 1353 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0019901; F:protein kinase binding; IDA:MGI.
 GO:0030292; F:protein tyrosine kinase inhibitor activity; IDA:MGI.
 GO:0051209; P:release of sequestered calcium ion into cytosol; IDA:MGI. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR009091; RCC1/BLIP-II.
 IPR000408; Reg_chr_condens. 
Pfam
 PF12796; Ank_2
 PF00651; BTB
 PF00415; RCC1 
SMART
 SM00248; ANK
 SM00225; BTB 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50097; BTB
 PS00625; RCC1_1
 PS00626; RCC1_2
 PS50012; RCC1_3 
PRINTS