CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000798
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Origin recognition complex subunit 5 
Protein Synonyms/Alias
  
Gene Name
 ORC5 
Gene Synonyms/Alias
 ORC5L 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
107NDFVRLFKQVTTAENubiquitination[1]
116VTTAENLKDQTVYIVubiquitination[2]
126TVYIVLDKAEYLRDMubiquitination[2, 3]
235LAVLNFPKYCEPVVKubiquitination[4]
242KYCEPVVKGEASERDubiquitination[4]
262RNIEPHLKKAMQTVYubiquitination[4]
263NIEPHLKKAMQTVYLubiquitination[4]
280ISSSQWEKLQKDDTDubiquitination[2, 4]
283SQWEKLQKDDTDPGQubiquitination[2]
292DTDPGQLKGLSAHTHubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. 
Sequence Annotation
 NP_BIND 37 44 ATP (Potential).  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; DNA replication; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 435 AA 
Protein Sequence
MPHLENVVLC RESQVSILQS LFGERHHFSF PSIFIYGHTA SGKTYVTQTL LKTLELPHVF 60
VNCVECFTLR LLLEQILNKL NHLSSSEDGC STEITCETFN DFVRLFKQVT TAENLKDQTV 120
YIVLDKAEYL RDMEANLLPG FLRLQELADR NVTVLFLSEI VWEKFRPNTG CFEPFVLYFP 180
DYSIGNLQKI LSHDHPPEYS ADFYAAYINI LLGVFYTVCR DLKELRHLAV LNFPKYCEPV 240
VKGEASERDT RKLWRNIEPH LKKAMQTVYL REISSSQWEK LQKDDTDPGQ LKGLSAHTHV 300
ELPYYSKFIL IAAYLASYNP ARTDKRFFLK HHGKIKKTNF LKKHEKTSNH LLGPKPFPLD 360
RLLAILYSIV DSRVAPTANI FSQITSLVTL QLLTLVGHDD QLDGPKYKCT VSLDFIRAIA 420
RTVNFDIIKY LYDFL 435 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005664; C:nuclear origin of replication recognition complex; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003688; F:DNA replication origin binding; TAS:ProtInc.
 GO:0000166; F:nucleotide binding; TAS:ProtInc.
 GO:0006270; P:DNA replication initiation; TAS:ProtInc.
 GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:Reactome. 
Interpro
 IPR020796; ORC5.
 IPR027417; P-loop_NTPase. 
Pfam
  
SMART
  
PROSITE
  
PRINTS