CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-040771
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ras GTPase-activating protein 3 
Protein Synonyms/Alias
 cDNA FLJ58396 
Gene Name
 RASA3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
72IQKEDLQKYHNRDTWubiquitination[1]
114DTGVVCHKLATRIVEubiquitination[1]
205LWNASNLKFGDEFLGubiquitination[1]
219GELRIPLKVLRQSSSubiquitination[1, 2]
244DNGSKSLKPDDLGSLubiquitination[2]
277PLRDLLLKSADVEPVubiquitination[1, 3]
346RGNSLASKCIDETMKubiquitination[1, 3]
609KLEEESFKMKNMFQVubiquitination[1, 2, 3, 4, 5]
714LYMSKLEKMQEACGSubiquitination[1, 2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; GTPase activation; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 802 AA 
Protein Sequence
MRDCYCTVNL DQEEVFRTKI VEKSLCPFYG EDFYCEIPRS FRHLSFYIFD RDVFRRDSII 60
GKVAIQKEDL QKYHNRDTWF QLQHVDADSE VQGKVHLELR LSEVITDTGV VCHKLATRIV 120
ECQGLPIVNG QCDPYATVTL AGPFRSEAKK TKVKRKTNNP QFDEVFYFEV TRPCSYSKKS 180
HFDFEEEDVD KLEIRVDLWN ASNLKFGDEF LGELRIPLKV LRQSSSYEAW YFLQPRDNGS 240
KSLKPDDLGS LRLNVVYTED HVFSSDYYSP LRDLLLKSAD VEPVSASAAH ILGEVCREKQ 300
EAAVPLVRLF LHYGRVVPFI SAIASAEVKR TQDPNTIFRG NSLASKCIDE TMKLAGMHYL 360
HVTLKPAIEE ICQSHKPCEI DPVKLKDGEN LENNMENLRQ YVDRVFHAIT ESGVSCPTVM 420
CDIFFSLREA AAKRFQDDPD VRYTAVSSFI FLRFFAPAIL SPNLFQLTPH HTDPQTSRTL 480
TLISKTVQTL GSLSKSKSAS FKESYMATFY EFFNEQKYAD AVKNFLDLIS SSGRRDPKSV 540
EQPIVLKEGF MIKRAQGRKR FGMKNFKKRW FRLTNHEFTY HKSKGDQPLY SIPIENILAV 600
EKLEEESFKM KNMFQVIQPE RALYIQANNC VEAKDWIDIL TKVSQCNQKR LTVYHPSAYL 660
SGHWLCCRAP SDSAPGCSPC TGGLPANIQL DIDGDRETER IYSLFNLYMS KLEKMQEACG 720
SKSVYDGPEQ EEYSTFVIDD PQETYKTLKQ VIAGVGALEQ EHAQYKRDKF KKTKYGSQEH 780
PIGDKSFQNY IRQQSETSTH SI 802 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR001936; RasGAP.
 IPR023152; RasGAP_CS.
 IPR008936; Rho_GTPase_activation_prot.
 IPR001562; Znf_Btk_motif. 
Pfam
 PF00779; BTK
 PF00168; C2
 PF00169; PH
 PF00616; RasGAP 
SMART
 SM00107; BTK
 SM00239; C2
 SM00233; PH
 SM00323; RasGAP 
PROSITE
 PS50004; C2
 PS50003; PH_DOMAIN
 PS00509; RAS_GTPASE_ACTIV_1
 PS50018; RAS_GTPASE_ACTIV_2
 PS51113; ZF_BTK 
PRINTS
 PR00402; TECBTKDOMAIN.