UniProt Accession

 COMT_MOUSE; O88587; Q91XH4 

Genbank Protein ID

 AF076156; AK148311; AC133487; BC010402 

Genbank Nucleotide ID

 AAC33334.1; BAE28473.1; AAH10402.1 

Protein Name

 Catechol O-methyltransferase 

Protein Synonyms/Alias

  

Gene Name

 Comt 

Gene Synonyms/Alias

 Comt1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
61	RHVQQHAKPGDPQSV	ubiquitination	[1]
170	QDLIPQLKKKYDVDT	ubiquitination	[1]
171	DLIPQLKKKYDVDTL	ubiquitination	[1]
172	LIPQLKKKYDVDTLD	ubiquitination	[1]
187	MVFLDHWKDRYLPDT	ubiquitination	[1]
205	EECGLLRKGTVLLAD	ubiquitination	[1]
252	KVVDGLEKAVYQGPG	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. 

Sequence Annotation

 REGION 160 163 S-adenosyl-L-methionine binding (By
 METAL 184 184 Magnesium (By similarity).
 METAL 212 212 Magnesium (By similarity).
 METAL 213 213 Magnesium (By similarity).
 BINDING 85 85 S-adenosyl-L-methionine; via amide
 BINDING 115 115 S-adenosyl-L-methionine (By similarity).
 BINDING 133 133 S-adenosyl-L-methionine (By similarity).
 BINDING 184 184 S-adenosyl-L-methionine (By similarity).
 BINDING 187 187 Substrate (By similarity).
 BINDING 213 213 Substrate (By similarity).
 BINDING 242 242 Substrate (By similarity).
 MOD_RES 261 261 Phosphoserine.  

Keyword

 Alternative initiation; Catecholamine metabolism; Cell membrane; Complete proteome; Cytoplasm; Magnesium; Membrane; Metal-binding; Methyltransferase; Neurotransmitter degradation; Phosphoprotein; Reference proteome; S-adenosyl-L-methionine; Signal-anchor; Transferase; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 265 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0016206; F:catechol O-methyltransferase activity; IDA:MGI.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0006584; P:catecholamine metabolic process; IEA:UniProtKB-KW.
 GO:0042420; P:dopamine catabolic process; IDA:MGI.
 GO:0008210; P:estrogen metabolic process; IEA:Compara.
 GO:0007565; P:female pregnancy; IEA:Compara.
 GO:0007612; P:learning; IEA:Compara.
 GO:0048609; P:multicellular organismal reproductive process; IEA:Compara.
 GO:0045963; P:negative regulation of dopamine metabolic process; IEA:Compara.
 GO:0048662; P:negative regulation of smooth muscle cell proliferation; IEA:Compara.
 GO:0042135; P:neurotransmitter catabolic process; IEA:UniProtKB-KW.
 GO:0050668; P:positive regulation of homocysteine metabolic process; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0032496; P:response to lipopolysaccharide; IEA:Compara.
 GO:0014070; P:response to organic cyclic compound; IEA:Compara.
 GO:0048265; P:response to pain; IEA:Compara. 

Interpro

 IPR025782; Catechol_O-MeTrfase.
 IPR017128; Catechol_O-MeTrfase_euk.
 IPR002935; O-MeTrfase_3. 

Pfam

 PF01596; Methyltransf_3 

SMART

  

PROSITE

  

PRINTS