CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021811
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 
Protein Synonyms/Alias
  
Gene Name
 Hcn2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
150LLQPGVNKFSLRMFGubiquitination[1]
160LRMFGSQKAVEREQEubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Can also transport ammonium in the distal nephron. Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages (By similarity). 
Sequence Annotation
 NP_BIND 517 634 cAMP.
 REGION 131 182 Involved in subunit assembly (By
 MOD_RES 119 119 Phosphoserine (By similarity).
 MOD_RES 641 641 Phosphoserine; by PKG/PRKG2 (By
 MOD_RES 750 750 Phosphoserine (By similarity).
 MOD_RES 771 771 Phosphoserine (By similarity).
 CARBOHYD 380 380 N-linked (GlcNAc...) (Potential).  
Keyword
 cAMP; cAMP-binding; Complete proteome; Glycoprotein; Ion channel; Ion transport; Ligand-gated ion channel; Membrane; Nucleotide-binding; Phosphoprotein; Potassium; Potassium channel; Potassium transport; Reference proteome; Sodium; Sodium channel; Sodium transport; Transmembrane; Transmembrane helix; Transport; Voltage-gated channel. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 863 AA 
Protein Sequence
MDARGGGGRP GDSPGTTPAP GPPPPPPPPA PLQLQPPPAP PPNPTTPSHP ESADEPGPRS 60
RLCSRDSSCT PGAAKGGANG ECGRGEPQCS PEGPARGPKV SFSCRGAASG PAAAEEAGSE 120
EAGPAGEPRG SQASFLQRQF GALLQPGVNK FSLRMFGSQK AVEREQERVK SAGAWIIHPY 180
SDFRFYWDFT MLLFMVGNLI IIPVGITFFK DETTAPWIVF NVVSDTFFLM DLVLNFRTGI 240
VIEDNTEIIL DPEKIKKKYL RTWFVVDFVS SIPVDYIFLI VEKGIDSEVY KTARALRIVR 300
FTKILSLLRL LRLSRLIRYI HQWEEIFHMT YDLASAVMRI CNLISMMLLL CHWDGCLQFL 360
VPMLQDFPSD CWVSINNMVN HSWSELYSFA LFKAMSHMLC IGYGRQAPES MTDIWLTMLS 420
MIVGATCYAM FIGHATALIQ SLDSSRRQYQ EKYKQVEQYM SFHKLPADFR QKIHDYYEHR 480
YQGKMFDEDS ILGELNGPLR EEIVNFNCRK LVASMPLFAN ADPNFVTAML TKLKFEVFQP 540
GDYIIREGTI GKKMYFIQHG VVSVLTKGNK EMKLSDGSYF GEICLLTRGR RTASVRADTY 600
CRLYSLSVDN FNEVLEEYPM MRRAFETVAI DRLDRIGKKN SILLHKVQHD LSSGVFNNQE 660
NAIIQEIVKY DREMVQQAEL GQRVGFFPPP PPRQVRSAIA TLQQAVAMSF CPQVARPLVG 720
PLALGSPRLV RRAPPGPLPP AASPGPPAAS PPAAPSSPRA PRTSPYGVPG SPATRVGPAL 780
PARRLSRASR PLSASQPSLP HGAPAPSPAA SARPASSSTP RLGPAPTTRT AAPSPDRRDS 840
ASPGAASGLD PLDSARSRLS SNL 863 
Gene Ontology
 GO:0030424; C:axon; IDA:RGD.
 GO:0043198; C:dendritic shaft; IDA:RGD.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0043025; C:neuronal cell body; IDA:RGD.
 GO:0005886; C:plasma membrane; IDA:RGD.
 GO:0045202; C:synapse; IDA:RGD.
 GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
 GO:0032947; F:protein complex scaffold; IDA:RGD.
 GO:0005272; F:sodium channel activity; IEA:UniProtKB-KW.
 GO:0005249; F:voltage-gated potassium channel activity; IEA:InterPro.
 GO:0007568; P:aging; IEP:RGD.
 GO:0007420; P:brain development; IEP:RGD.
 GO:0045823; P:positive regulation of heart contraction; IMP:RGD.
 GO:0042493; P:response to drug; IEP:RGD.
 GO:0009725; P:response to hormone stimulus; IEP:RGD.
 GO:0035725; P:sodium ion transmembrane transport; IEA:GOC. 
Interpro
 IPR018490; cNMP-bd-like.
 IPR018488; cNMP-bd_CS.
 IPR000595; cNMP-bd_dom.
 IPR005821; Ion_trans_dom.
 IPR013621; Ion_trans_N.
 IPR003938; K_chnl_volt-dep_EAG/ELK/ERG.
 IPR014710; RmlC-like_jellyroll. 
Pfam
 PF00027; cNMP_binding
 PF00520; Ion_trans
 PF08412; Ion_trans_N 
SMART
 SM00100; cNMP 
PROSITE
 PS00888; CNMP_BINDING_1
 PS00889; CNMP_BINDING_2
 PS50042; CNMP_BINDING_3 
PRINTS
 PR01463; EAGCHANLFMLY.