CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004855
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Muscarinic acetylcholine receptor M3 
Protein Synonyms/Alias
  
Gene Name
 CHRM3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
412ADKLQAQKSVDDGGSubiquitination[1]
426SFPKSFSKLPIQLESubiquitination[1]
439ESAVDTAKTSDVNSSubiquitination[1]
449DVNSSVGKSTATLPLubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. 
Sequence Annotation
 MOTIF 275 281 Basolateral sorting signal.
 CARBOHYD 5 5 N-linked (GlcNAc...) (Potential).
 CARBOHYD 6 6 N-linked (GlcNAc...) (Potential).
 CARBOHYD 15 15 N-linked (GlcNAc...) (Potential).
 CARBOHYD 41 41 N-linked (GlcNAc...) (Potential).
 CARBOHYD 48 48 N-linked (GlcNAc...) (Potential).
 DISULFID 141 221 By similarity.  
Keyword
 3D-structure; Cell junction; Cell membrane; Complete proteome; Disulfide bond; G-protein coupled receptor; Glycoprotein; Membrane; Polymorphism; Postsynaptic cell membrane; Receptor; Reference proteome; Synapse; Transducer; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 590 AA 
Protein Sequence
MTLHNNSTTS PLFPNISSSW IHSPSDAGLP PGTVTHFGSY NVSRAAGNFS SPDGTTDDPL 60
GGHTVWQVVF IAFLTGILAL VTIIGNILVI VSFKVNKQLK TVNNYFLLSL ACADLIIGVI 120
SMNLFTTYII MNRWALGNLA CDLWLAIDYV ASNASVMNLL VISFDRYFSI TRPLTYRAKR 180
TTKRAGVMIG LAWVISFVLW APAILFWQYF VGKRTVPPGE CFIQFLSEPT ITFGTAIAAF 240
YMPVTIMTIL YWRIYKETEK RTKELAGLQA SGTEAETENF VHPTGSSRSC SSYELQQQSM 300
KRSNRRKYGR CHFWFTTKSW KPSSEQMDQD HSSSDSWNNN DAAASLENSA SSDEEDIGSE 360
TRAIYSIVLK LPGHSTILNS TKLPSSDNLQ VPEEELGMVD LERKADKLQA QKSVDDGGSF 420
PKSFSKLPIQ LESAVDTAKT SDVNSSVGKS TATLPLSFKE ATLAKRFALK TRSQITKRKR 480
MSLVKEKKAA QTLSAILLAF IITWTPYNIM VLVNTFCDSC IPKTFWNLGY WLCYINSTVN 540
PVCYALCNKT FRTTFKMLLL CQCDKKKRRK QQYQQRQSVI FHKRAPEQAL 590 
Gene Ontology
 GO:0032279; C:asymmetric synapse; IEA:Compara.
 GO:0043679; C:axon terminus; IEA:Compara.
 GO:0016323; C:basolateral plasma membrane; IEA:UniProtKB-SubCell.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
 GO:0042166; F:acetylcholine binding; IEA:Compara.
 GO:0008144; F:drug binding; IEA:Compara.
 GO:0016907; F:G-protein coupled acetylcholine receptor activity; TAS:ProtInc.
 GO:0004435; F:phosphatidylinositol phospholipase C activity; TAS:ProtInc.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0006464; P:cellular protein modification process; TAS:ProtInc.
 GO:0007586; P:digestion; IEA:Compara.
 GO:0006112; P:energy reserve metabolic process; TAS:Reactome.
 GO:0007399; P:nervous system development; TAS:ProtInc.
 GO:0045987; P:positive regulation of smooth muscle contraction; IEA:Compara.
 GO:0050796; P:regulation of insulin secretion; TAS:Reactome.
 GO:0043269; P:regulation of ion transport; IEA:InterPro.
 GO:1900274; P:regulation of phospholipase C activity; IEA:InterPro.
 GO:0003056; P:regulation of vascular smooth muscle contraction; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0006939; P:smooth muscle contraction; IEA:Compara. 
Interpro
 IPR000276; GPCR_Rhodpsn.
 IPR017452; GPCR_Rhodpsn_7TM.
 IPR001183; Musac_Ach_M3_rcpt.
 IPR000995; Musac_Ach_rcpt. 
Pfam
 PF00001; 7tm_1 
SMART
  
PROSITE
 PS00237; G_PROTEIN_RECEP_F1_1
 PS50262; G_PROTEIN_RECEP_F1_2 
PRINTS
 PR00237; GPCRRHODOPSN.
 PR00243; MUSCARINICR.
 PR00540; MUSCRINICM3R.