CPLM-014601

Genbank Nucleotide ID

Serine/threonine-protein kinase PAK 2

Protein Synonyms/Alias

Gamma-PAK; p21-activated kinase 2; PAK-2; PAK-2p27; PAK-2p34

Rattus norvegicus (Rat)

Position	Peptide	Type	References
38	LSANHSLKPLPSVPE	acetylation	[1]
226	DKQKKKAKMTDEEIM	acetylation	[1]
370	QVIHRDIKSDNVLLG	ubiquitination	[2]

[1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
[2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]

Functional Description

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF- induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase- activated PAK2 phosphorylates MKNK1 and reduces cellular translation (By similarity).

DOMAIN 74 87 CRIB.
DOMAIN 249 500 Protein kinase.
NP_BIND 255 263 ATP (By similarity).
REGION 69 137 Autoregulatory region (By similarity).
REGION 69 112 GTPase-binding (By similarity).
REGION 88 248 Linker.
MOTIF 245 251 Nuclear localization signal (By
ACT_SITE 368 368 Proton acceptor (By similarity).
BINDING 278 278 ATP (By similarity).
MOD_RES 2 2 N-acetylserine (By similarity).
MOD_RES 2 2 Phosphoserine (By similarity).
MOD_RES 58 58 Phosphoserine (By similarity).
MOD_RES 128 128 N6-acetyllysine (By similarity).
MOD_RES 141 141 Phosphoserine.
MOD_RES 143 143 Phosphothreonine (By similarity).
MOD_RES 169 169 Phosphothreonine (By similarity).
MOD_RES 197 197 Phosphoserine (By similarity).
MOD_RES 402 402 Phosphothreonine; by autocatalysis (By

Acetylation; Allosteric enzyme; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Lipoprotein; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.

IPR011009; Kinase-like_dom.
IPR000095; PAK_box_Rho-bd.
IPR000719; Prot_kinase_cat_dom.
IPR017441; Protein_kinase_ATP_BS.
IPR002290; Ser/Thr_dual-sp_kinase_dom.
IPR008271; Ser/Thr_kinase_AS.

PF00786; PBD
PF00069; Pkinase

SM00285; PBD
SM00220; S_TKc

PS50108; CRIB
PS00107; PROTEIN_KINASE_ATP
PS50011; PROTEIN_KINASE_DOM
PS00108; PROTEIN_KINASE_ST